We narrowed to 22,374 results for: ESC;

1. pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

   Plasmid

   #218882

   Purpose

   AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2(no bind)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   CAG

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-CjCas9-MS2gRNA-Backbone

   Plasmid

   #210711

   Purpose

   This plasmid express MS2 loop containing Cj Cas9 gRNA

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MS2 loop containing Cj Cas9 gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVRa41_491

   Plasmid

   #49702

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF41_491
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-FlaviA-GFP-puro

   Plasmid

   #140088

   Purpose

   Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   FlaviA-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDuex2-iBB-GECO1

   Plasmid

   #194216

   Purpose

   Far-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.

   Depositor

   Robert Campbell

   Article

   Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

   Insert

   iBB-GECO1
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-surrogate-reporter

   Plasmid

   #65624

   Purpose

   Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attP
   

   Use

   Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn7-133

   Plasmid

   #118551

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn7 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn5-153

   Plasmid

   #118535

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2-7xTcf-NLS-CNL

   Plasmid

   #65712

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL
   

   Use

   Luciferase

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRE-Tn5-150

    Plasmid

    #118532

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mito-mEosEM-E

    Plasmid

    #226335

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    COX8A (COX8A Human)
    

    Tags

    mEosEM-E

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKD072

    Plasmid

    #136472

    Purpose

    SSB-C-term-GFP fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-C-term-GFP (ssb E. coli)
    

    Tags

    superfolder GFP

    Expression

    Bacterial

    Mutation

    superfolder GFP attached to C-terminus at Phe 178

    Promoter

    T7 promoter

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. NPY-mApple

    Plasmid

    #83498

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE-Tn7-150

    Plasmid

    #118564

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

    Plasmid

    #113890

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn5-131

    Plasmid

    #118517

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

    Plasmid

    #199585

    Purpose

    Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
    AkaLuc
    Blasticidin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCS.71/2272.Hygro

    Plasmid

    #22731

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox71-FRT-PGK-hygro-FRT-Lox2272
    

    Use

    Cre/Lox

    Mutation

    This is a basal exchange vector for RMCE. The vec…

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRE-Tn5-130

    Plasmid

    #118516

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid

    #100018

    Purpose

    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpsfGFP-LSSmOrange
    

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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