We narrowed to 3,760 results for: biorxiv

1. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #223173

   Purpose

   Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hfCas13d (N2V8)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB_r5-HT1.0

   Plasmid

   #208719

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

   Plasmid

   #230988

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-GFAP-iGABASnFR2-WPRE

   Plasmid

   #218871

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   GFAP

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-syn-iGABASnFR2n-WPRE

   Plasmid

   #218875

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   Synapsin

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPolI_SARS-CoV-2_NLuc

   Plasmid

   #183953

   Purpose

   Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

   Depositor

   AJ te Velthuis

   Article

   Vial et al bioRxiv 2021.06.28.450211

   Insert

   SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mammalian Pol I

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mito-CanlonicSF/pCMV-myc-mito

   Plasmid

   #224465

   Purpose

   To explore mitochondrial lactate dynamics

   Depositor

   Alejandro San Martín

   Article

   Rauseo et al bioRxiv 2024.07.19.604361

   Insert

   CanlonicSF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

   Plasmid

   #223175

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   DR1 A>T

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TetON-Bmpr1A-ca

    Plasmid

    #217536

    Purpose

    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    constitutively active BMP receptor 1a (BMPR1A Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet operon (TetO)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-DRP1 K38A

    Plasmid

    #232350

    Purpose

    Expresses K38A variant of human DRP1 with mCherry tag

    Depositor

    Samantha Lewis

    Article

    Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

    Insert

    DRP1 (DNM1L Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDisplay-GRAB_r5-HT1.0

    Plasmid

    #208713

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_rtTA-P2A-BFP_HygroR

    Plasmid

    #186738

    Purpose

    rtTA-P2A-BFP expression construct cloned into lentiviral backbone

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GfaABC1D-GRAB_g5-HT3.0

    Plasmid

    #208727

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid

    #208573

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    d2nucEGFP
    HygroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV and minP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

    Plasmid

    #194217

    Purpose

    Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    UAS-ybTATA-tagBFP; PGK-mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GAL4 UAS-ybTATA; PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

    Plasmid

    #216533

    Purpose

    An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

    Depositor

    Yuki Kambe

    Article

    Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

    Insert

    axon-jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_EZH2gRNA14

    Plasmid

    #199586

    Purpose

    Contains guide RNA to 5' end of mouse EZH2 gene

    Depositor

    Mauro Calabrese

    Article

    Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

    Insert

    EZH2 (Ezh2 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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