Addgene

We narrowed to 11,654 results for: cel.2

Showing: 4411 - 4440 of 11654 results

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PHR-GFP-Rta

Plasmid

#183930

Purpose

Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-Rta (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VPR

Plasmid

#183928

Purpose

Optogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VPR (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SOX2

Plasmid

#35388

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Sox2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pCMVMagFRET-6-NLS

Plasmid

#50757

Purpose

Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-6 with NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-OCT4

Plasmid

#35391

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Oct4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2012

Availability

Academic Institutions and Nonprofits only
pCB6-ZONAB-B

Plasmid

#136642

Purpose

Expression of ZONAB isoform B in mammalian cells

Depositor

Karl Matter, Maria Balda

Article

Balda et al EMBO J. 2000 May 2;19(9):2024-33. doi: 1

Insert

ZONAB isoform B (YBX3 Canine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1-SNAP

Plasmid

#223576

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PD-1

Plasmid

#223572

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-PD-L1-SNAP

Plasmid

#223577

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-PD-L1

Plasmid

#223571

Purpose

Lentiviral expression of human PD-L1 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116

Plasmid

#123105

Purpose

Expresses 3xFLAG-GABARAPL2 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING (1-339)

Plasmid

#229740

Purpose

Expresses mouse STING amino acids 1-339 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Use

Tags

None

Expression

Mammalian

Mutation

Contains amino acids 1-339

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-PD-1

Plasmid

#223569

Purpose

Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Tags

Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PD-1 I126A

Plasmid

#223573

Purpose

Lentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

I126A

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2:2A:Ascl1

Plasmid

#127289

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

Depositor

Fred Gage

Article

Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

Inserts

Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and Ascl1 linked via 2A

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-1

Plasmid

#50742

Purpose

Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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HA-hNPHP1

Plasmid

#122872

Purpose

Expresses HA-tagged human NPHP1 in mammalian cells

Depositor

Ken-Ichi Takemaru, FENG-QIAN Li

Article

Hoque et al Reproduction. 2021 Jul 8;162(2):129-139.

Insert

human NPHP1 (NPHP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pNC-Green-SubC

Plasmid

#193407

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-Green-SubN

Plasmid

#193406

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EF1a-KLF4

Plasmid

#35389

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Klf4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pLentiN 6bE6

Plasmid

#37447

Purpose

Lentiviral expression of HPV 6bE6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 6bE6 (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pLentiN 5E6

Plasmid

#37449

Purpose

Lentiviral expression of HPV 5E6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 5E6 (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pNC-RFP-N

Plasmid

#193412

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-RFP-C

Plasmid

#193413

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-mCherry-N

Plasmid

#193414

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-mCherry-C

Plasmid

#193415

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-CFP-N

Plasmid

#193410

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-121-SubC

Plasmid

#193409

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-Cam1304-SubN

Plasmid

#193404

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge