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mCh-sspB(LOV2)

Plasmid

#223689

Purpose

PhoBIT1 component; mCherry-tagged sspB with LOV2 insertion

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

sspB(N)-LOV2-sspB(C)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iC

Plasmid

#231418

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iA

Plasmid

#231417

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE

Plasmid

#231804

Purpose

Label centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.

Depositor

Annette Oxenius

Insert

Kaede-CETN1 (Cetn1 Mouse)

Use

Lentiviral

Tags

Kaede

Expression

Mammalian

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP65

Plasmid

#196803

Purpose

Contains ATG8A promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

AUTOPHAGY-RELATED 8A

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP72

Plasmid

#196810

Purpose

Contains PMR4 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

POWDERY MILDEW RESISTANT 4

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#226834

Purpose

Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

Olactb and U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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POC1486

Plasmid

#213811

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M2.HpyAII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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POC1464

Plasmid

#213736

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1481

Plasmid

#213807

Purpose

LguI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

LguI-associated switch methylase M.XmnI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1474

Plasmid

#213762

Purpose

BpiI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated switch methylase M2.NmeMC58II

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1488

Plasmid

#213813

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.MboII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1479

Plasmid

#213805

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1480

Plasmid

#213806

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M1.Eco31I

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1484

Plasmid

#213810

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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