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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 2941 - 2960 of 21629 results

  1. pAAV GFP

    Plasmid
    #213685
    Purpose
    Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Twin-Strep GFP
    Use
    AAV
    Tags
    Twin-Strep
    Expression
    Mutation
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213685-plasmid-map-sequence-id-455753

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  2. pDM097

    Plasmid
    #216819
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216819-plasmid-map-sequence-id-430144

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  9. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  10. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  12. pASPIre4

    Plasmid
    #196656
    Purpose
    Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    SpeI site in Bxb1 CDS
    Promoter
    rhamnose-inducible promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196656-plasmid-map-sequence-id-403168

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  14. pTP-PCR C3

    Plasmid
    #189620
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Removed repeat region, 10-kbp deletion, and minor…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189620-plasmid-map-sequence-id-379033

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  18. pPEE42

    Plasmid
    #139421
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    Citrine
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    139421-plasmid-map-sequence-id-324126

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  19. pPEE45

    Plasmid
    #139424
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mTurquoise1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139424-plasmid-map-sequence-id-313811

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  20. pPEE44

    Plasmid
    #139423
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mTFP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139423-plasmid-map-sequence-id-313810

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Showing: 2941 - 2960 of 21629 results