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We narrowed to 148,842 results for: ins;

Showing: 2941 - 2960 of 148842 results

  2. pET-30a(+)-Syk-tSH2-FX

    Plasmid
    #111272
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
    Expression
    Bacterial
    Mutation
    interdomain A (Phe 119 to His 162) substituted by…
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111272-plasmid-map-sequence-id-215700

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  5. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

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  7. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid
    #162278
    Purpose
    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN1A Variant 1, stabilized (SCN1A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp2946. Silent codon c…
    Promoter
    CMV
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162278-plasmid-map-sequence-id-338003

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  17. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid
    #105802
    Purpose
    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-mRuby3; ORF2: TEV-mClover3
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105802-plasmid-map-sequence-id-203396

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  18. pEW108

    Plasmid
    #232823
    Purpose
    Expresses yeast compass complex in insect cells
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    Set1(762-1080)
    Promoter
    polyhedrin promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232823-plasmid-map-sequence-id-465728

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Showing: 2941 - 2960 of 148842 results