We narrowed to 17,207 results for: puro

1. pZIP-hCMV-ZsGreen-P2A-Puro-NT4

   Plasmid

   #123328

   Purpose

   Lentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   non-targeting control shRNA embedded in miR30 backbone
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

   Plasmid

   #176892

   Purpose

   Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Article

   Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

   Insert

   moxGFP-P2A-T2A-c-Myc
   

   Use

   Transposon

   Tags

   Myc and moxGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. N111 nLV EF-1a-PGK-Puro Gal4-HP1a FL

   Plasmid

   #43968

   Depositor

   Jerry Crabtree

   Article

   Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

   Insert

   Cbx5 (Cbx5 Mouse)
   

   Use

   Lentiviral

   Tags

   Gal4/DBD

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   June 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Flag-Polß(D256A)-IRES-puro

   Plasmid

   #128677

   Purpose

   mammalian expression of Flag-Polß(D256A) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D256A

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

   Plasmid

   #75123

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   3x FLAG-tag

   Expression

   Mutation

   bromodomain replaced with the first bromodomain o…

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

6. MSCV-6NBC-GFP-PGK-Puro-Barcode1

   Plasmid

   #83678

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode1
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

   Plasmid

   #22023

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450A.Rac1 s…

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

   Plasmid

   #75118

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Bromodomain replaced with the bromodomain of BRD7

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pBabe puro-HA-PIK3CA(H1047R;F486S;del)

   Plasmid

   #33255

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and phenylalan…

   Promoter

   SV40

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid

    #138525

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    Control non-PRC2 binding A-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

    Plasmid

    #110830

    Purpose

    Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

    Depositor

    Ande Satyanarayana

    Article

    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

    Insert

    AZGP1 (Azgp1 Mouse)
    

    Use

    Non-viral gene expression vector

    Tags

    T2A and dTomato

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV Puro DEST (w118-1) VOPP1

    Plasmid

    #107507

    Purpose

    expressing VOPP1, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    VOPP1 (VOPP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

    Plasmid

    #203439

    Purpose

    Mammalian expression of RUNX4

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    RUNX3 (RUNX3 Human)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    R122C

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-C-Myc-DDK-IRES-Puro HNRNPR

    Plasmid

    #200730

    Purpose

    Lentiviral expression of Flag-Tagged human HNRNPR

    Depositor

    Claudio Alarcón

    Insert

    HNRNPR (HNRNPR Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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