We narrowed to 162,536 results for: addgene

1. pMVRha

   Plasmid

   #229553

   Purpose

   Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

   Depositor

   Sandy Schmidt

   Article

   Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGFP-AUA-IRES-mCherry

   Plasmid

   #222116

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   AUA start codon

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGFP-AGC-IRES-mCherry

   Plasmid

   #222119

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   AGC start codon

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZB260_pT7-STR-H3U3

   Plasmid

   #228506

   Purpose

   Bacterial Hybrid Reporter Plasmid, pT7-HIS3/URA3

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   HIS3/URA3 operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP-CUG-IRES-mCherry

   Plasmid

   #222110

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   CUG start codon

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGFP-ACG-IRES-mCherry

   Plasmid

   #222113

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ACG start codon

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGFP-UUG-IRES-mCherry

   Plasmid

   #222112

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   UUG start codon

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGFP-AUU-IRES-mCherry

   Plasmid

   #222118

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   AUU start codon

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGFP-AGG-IRES-mCherry

   Plasmid

   #222115

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   AGG start codon

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFP-AUC-IRES-mCherry

    Plasmid

    #222117

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    AUC start codon

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGAL4-sfGFP(1-157)

    Plasmid

    #220743

    Purpose

    GAL4-sfGFP(1-157) construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-sfGFP(1-157)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAL4-sfGFP(158-238)

    Plasmid

    #220744

    Purpose

    GAL4-sfGFP(158-238) construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-sfGFP(158-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAL4-NIR-FbLAG30

    Plasmid

    #220746

    Purpose

    GAL4-NIR-FbLAG30 construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCL452

    Plasmid

    #219502

    Purpose

    Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL390

    Plasmid

    #219500

    Purpose

    Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
    

    Use

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-GAL10

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid

    #208645

    Purpose

    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

    Depositor

    Quanfu Ma

    Insert

    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    

    Use

    CRISPR; Ssdna donnor preparation for knock-in of…

    Tags

    Twin Strep

    Expression

    Mutation

    Two SNP (rs749555789, rs760194863) were introduce…

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMIGII-TCR-F

    Plasmid

    #194392

    Purpose

    Retroviral expression of candidate orphan TCR

    Depositor

    Michael Carroll

    Article

    Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

    Insert

    TCR-F (Tcra, Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIGII-TCR-G

    Plasmid

    #194393

    Purpose

    Retroviral expression of candidate orphan TCR

    Depositor

    Michael Carroll

    Article

    Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

    Insert

    TCR-G (Tcra, Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge