We narrowed to 5,882 results for: pcDNA 3.1

1. pTS2368-Tier1-PhCMV_VP64

   Plasmid

   #169569

   Purpose

   Tier-1 vector encoding PhCMV-driven VP64 transactivator domain (PhCMV-VP64-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVH237-2-Tier1-PmPGK1-TtgA

   Plasmid

   #169584

   Purpose

   Tier-1 vector encoding PmPGK1-driven TtgA expression (PmPGK1-TtgA-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Regulatory protein TtgR fused to a VP16 domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2367-Tier1-PhCMV_VP16

   Plasmid

   #169568

   Purpose

   Tier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS1011-Tier1-PhCMV-Nluc

   Plasmid

   #169545

   Purpose

   Tier-1 vector encoding PhCMV-driven NLuc (PhCMV-NLuc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven NanoLuc Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFOX8-Tier1-PhCMV-YPet

   Plasmid

   #169506

   Purpose

   Tier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2401-Tier1-PhCMV-HygroR

   Plasmid

   #169493

   Purpose

   Tier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven hph
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS1018-Tier1-PhCMV-p2A

   Plasmid

   #169491

   Purpose

   Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven 2A sequence of porcine teschovirus-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2415-Tier1-PPGK-1xEAAAK

   Plasmid

   #169482

   Purpose

   Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven EAAAK-linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2412-Tier1-PPGK-2xGS

   Plasmid

   #169481

   Purpose

   Tier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven GS-linker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniCMV-mCerulean-tDeg

    Plasmid

    #185401

    Purpose

    mCerulean-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    mCerulean-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

    Plasmid

    #182854

    Purpose

    Hetero-tetrmer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    myr-palm-mEYFP-mCherry2-mEGFP-mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lmajor_gp63_10_0460_D463N

    Plasmid

    #171644

    Purpose

    Expression of L. major glcyoprotein-63 (D463N) with substrate binding site mutation from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

    Insert

    LmjF.10.0460 D463N
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    D463N

    Promoter

    Available since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PA-Bongwoori-R3

    Plasmid

    #137838

    Purpose

    A photoactivatable GEPI variant originated from ecliptic pHluorin

    Depositor

    Bradley Baker

    Article

    Lee et al Front Cell Neurosci. 2019 Oct 29;13:482.

    Insert

    Photoactivatable_Bongwoori-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L64F, T65S, V163A, T203H, A227D

    Promoter

    CMV

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOVC1_X_HsPDZ1b

    Plasmid

    #131216

    Purpose

    Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    HsPDZ1b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOVC1_HsPDZ1b_X

    Plasmid

    #131214

    Purpose

    Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    HsPDZ1b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOVC1_X_AtPIF6

    Plasmid

    #131192

    Purpose

    Expresses AtPIF6 (Arabidopsis thaliana) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AtPIF6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CC1-Pos6

    Plasmid

    #101656

    Purpose

    CC1-Pos6 is a modified version of CC1-M240 that has 4 arginines in its linker. (* super ecliptic pHluorin A227D)

    Depositor

    Bradley Baker

    Article

    Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

    Insert

    CC1-Pos6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CC1-M240

    Plasmid

    #101657

    Purpose

    CC1 with shorter linker length and the 'GDP' fusion site. (* super ecliptic pHluorin A227D)

    Depositor

    Bradley Baker

    Article

    Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

    Insert

    CC1-M240
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmyc-GFP-TNRC6C-(928-1020)

    Plasmid

    #42026

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6C (TNRC6C Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa928-1020

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-tagged-SF1 (antisense)

    Plasmid

    #28183

    Depositor

    Bernard Schimmer

    Article

    Frigeri et al Mol Endocrinol. 2000 Apr . 14(4):535-44.

    Insert

    SF1 (Nr5a1 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Reverse Orientation

    Promoter

    Available since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

21. FRESCA-syntide

    Plasmid

    #132415

    Purpose

    FRET-based sensor for CaMKII activity

    Depositor

    Margaret Stratton

    Article

    Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

    Insert

    FRESCA-syntide
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Halo-KDEL

    Plasmid

    #124316

    Purpose

    Expresses ER specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    KDEL Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DYSF-3HA

    Plasmid

    #29767

    Depositor

    Steven Vogel

    Article

    Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

    Insert

    DYSF (DYSF Human)
    

    Use

    Tags

    3xHA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP- SEC61B

    Plasmid

    #121159

    Purpose

    Mammalian expression of GFP tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Vinculin-venus

    Plasmid

    #27300

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    Venus A206K

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST-ORF-V2

    Plasmid

    #73638

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. enOgeuIscB with ωRNA-v13

    Plasmid

    #220940

    Purpose

    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

    Inserts

    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mPiezo1-IRES-eGFP

    Plasmid

    #80925

    Purpose

    expresses mouse Piezo1 with GFP

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

    Insert

    mouse Piezo1 CDS (Piezo1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Human, Synthetic)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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