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Showing: 5921 - 5960 of 8360 results

  1. pFRT-TODestGFP_HuD

    Plasmid
    #65763
    Purpose
    expresses GFP-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65763-plasmid-map-sequence-id-115023

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  2. pTXB1-CII

    Plasmid
    #51566
    Purpose
    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cII
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51566-plasmid-map-sequence-id-78825

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  3. RHPMP132 (mEOS3.1-beta Actin)

    Plasmid
    #88836
    Purpose
    Localization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1
    Depositor
    Ricardo Henriques
    Insert
    beta-Actin (ACTB Human)
    Use
    Tags
    mEOS3.1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88836-plasmid-map-sequence-id-177523

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  4. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  5. RHPMP133 (mOrange2-beta Actin)

    Plasmid
    #88837
    Purpose
    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    beta-Actin (ACTB Human)
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88837-plasmid-map-sequence-id-177529

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  6. AAV-hACE2-cMYC-Flag

    Plasmid
    #158957
    Purpose
    Used for expression of human ACE2 with AAV packaging
    Depositor
    Akiko Iwasaki
    Insert
    Human ACE2 (ACE2 Human)
    Use
    Tags
    Flag and MYC
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    158957-plasmid-map-sequence-id-310391

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  7. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid
    #154398
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154398-plasmid-map-sequence-id-304591

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  8. pLEX307-FURIN-G418

    Plasmid
    #158462
    Purpose
    Constitutive lentiviral expression of human FURIN.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    FURIN (FURIN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158462-plasmid-map-sequence-id-308175

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  10. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid
    #80077
    Purpose
    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection
    Depositor
    Phillip Abbosh
    Inserts
    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    Use
    Retroviral
    Tags
    5' FLAG
    Expression
    Mammalian
    Mutation
    Y641F, R342R, N393N
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    80077-plasmid-map-sequence-id-151639

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  11. pMT-HA-STX1

    Plasmid
    #89310
    Purpose
    Expresses HA tagged syntaxin 1 in Drosophila cells
    Depositor
    Andrew Peden
    Insert
    STX1 (Syx1A Fly)
    Use
    Tags
    HA tag
    Expression
    Insect
    Mutation
    Promoter
    P-MT (Metallothionein)
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89310-plasmid-map-sequence-id-190170

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  12. pBabe-Kras S17N

    Plasmid
    #58899
    Purpose
    Expresses human Kras with a S17N mutation
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    S17N mutation
    Promoter
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    58899-plasmid-map-sequence-id-158643

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  13. pFRT-DestFLAGHA_HuB

    Plasmid
    #65758
    Purpose
    constitutively expresses FLAG-HA-tagged HuB in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. AURKB

    Plasmid
    #39119
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AURKB (AURKB Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pDONR221-APN

    Plasmid
    #158440
    Purpose
    Gateway Cloning compatible entry vector for the human APN gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    APN (ANPEP Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158440-plasmid-map-sequence-id-308255

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  16. pFRT-TODestGFP_HuB

    Plasmid
    #65761
    Purpose
    expresses GFP-tagged HuB in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65761-plasmid-map-sequence-id-115012

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  18. Hs.NRAS Q61R

    Plasmid
    #83179
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61R
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83179-plasmid-map-sequence-id-158142

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  19. 4H4H

    Plasmid
    #51314
    Depositor
    Cheryl Arrowsmith
    Insert
    4H4H (EHMT1 Human, 4H4H)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pFRT-TODestFLAGHA_HuD

    Plasmid
    #65757
    Purpose
    expresses FLAGHA-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65757-plasmid-map-sequence-id-111829

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  23. Hs.NRAS Q61L

    Plasmid
    #83178
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61L
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83178-plasmid-map-sequence-id-158141

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  26. AICSDP-116:PCNA-mEGFP

    Plasmid
    #170818
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA
    Depositor
    Allen Institute for Cell Science
    Insert
    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    June 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170818-plasmid-map-sequence-id-334731

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  27. MARS

    Plasmid
    #205232
    Purpose
    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Use
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205232-plasmid-map-sequence-id-404748

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  28. pNIC28-hKdm2b-CxxC-PHD

    Plasmid
    #49216
    Purpose
    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.
    Depositor
    Rob Klose
    Insert
    Kdm2b (KDM2B Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49216-plasmid-map-sequence-id-72771

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  29. pFRT-DestFLAGHA_HuD

    Plasmid
    #65760
    Purpose
    constitutively expresses FLAG-HA-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pMT-TAK1-V5-His

    Plasmid
    #63753
    Purpose
    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TGF-β activated kinase 1 (Tak1 Fly)
    Use
    Tags
    V5-His
    Expression
    Insect
    Mutation
    Promoter
    pMT
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63753-plasmid-map-sequence-id-106438

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  31. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Use
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

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  32. Hs.HRAS Q61R

    Plasmid
    #83188
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61R
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83188-plasmid-map-sequence-id-158151

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  33. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  34. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pLEX307-APN-G418

    Plasmid
    #158456
    Purpose
    Constitutive lentiviral expression of human APN.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    APN (ANPEP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158456-plasmid-map-sequence-id-308149

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  36. pDest eGFP CUGBP1

    Plasmid
    #61276
    Purpose
    Mammalian expression of EGFP-tagged human CUGBP1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    CUG-BP (CELF1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61276-plasmid-map-sequence-id-99587

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  37. pDONR221-FURIN

    Plasmid
    #158442
    Purpose
    Gateway Cloning compatible entry vector for the human FURIN gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    FURIN (FURIN Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158442-plasmid-map-sequence-id-308251

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  38. pAG416-GAL-hnRNPA1

    Plasmid
    #181710
    Purpose
    Galactose inducible expression of hnRNPA1
    Depositor
    Alejandro Chavez
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181710-plasmid-map-sequence-id-430953

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  39. NEK2

    Plasmid
    #39163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NEK2 (NEK2 Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    T175A inactivating mutant
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. Hs.HRAS G12D

    Plasmid
    #83183
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12D
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83183-plasmid-map-sequence-id-158146

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Showing: 5921 - 5960 of 8360 results