We narrowed to 10,632 results for: t7

1. pAM238

   Plasmid

   #226639

   Purpose

   S. cerevisiae ubiquitin

   Depositor

   Andreas Martin

   Article

   Lander et al Nature. 2012 Jan 11;482(7384):186-91. do

   Insert

   Ubiquitin (UBI4 Budding Yeast)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLz48

   Plasmid

   #226322

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   strep-CsoS1A
   

   Tags

   Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBz12

   Plasmid

   #226321

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-Rubisco
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLz92

   Plasmid

   #226317

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-CsoS2B
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-a(+)-6xHis-mBlueberry(-9)

   Plasmid

   #219416

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   mBlueberry2(-9)
   

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   K137E, K199E, R234E

   Promoter

   T7

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HisMS2_VLP_FluB

   Plasmid

   #175956

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza B NSP 1 protein [B/Colorado/06/2017]

   Depositor

   Paul Freemont

   Article

   Unruh et al medRxiv 2024.04.17.24305965

   Insert

   NEP gene
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pD(P+T)_T7N

   Plasmid

   #223855

   Purpose

   4G-cloning donor for bacterial promoter and terminator, N-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD(P+T)_T7C

   Plasmid

   #223856

   Purpose

   4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1-mCherry (FspAI) (JM65)

   Plasmid

   #206445

   Purpose

   Expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Insect

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-CdRdhA

    Plasmid

    #217824

    Purpose

    Expresses N-His-CdRdhA in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    N-His-CdRhdA
    

    Tags

    His6x

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDB179-RdhC1

    Plasmid

    #217822

    Purpose

    Expresses HisSUMO-RdhC1 in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    RdhC1
    

    Use

    Unspecified

    Tags

    His10xSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDB179-RdhF3

    Plasmid

    #217823

    Purpose

    Expresses HisSUMO-RdhF3 in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    RdhF3
    

    Tags

    His10xSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-DhFld

    Plasmid

    #217821

    Purpose

    Expresses N-His-DhFld in E. coli

    Depositor

    Pamela Silver

    Article

    Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

    Insert

    DhFld
    

    Tags

    His6x

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-mEGFP(A206K)-TwStrep

    Plasmid

    #208622

    Purpose

    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone

    Depositor

    Quanfu Ma

    Insert

    mEGFP(A206K)
    

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    (compared with pEGFP-C1, protein level) A206K

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid

    #164055

    Purpose

    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. mouse KANK4 Ankyrin Repeats pET151

    Plasmid

    #164057

    Purpose

    Expresses mouse KANK4 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b_rHCaMP

    Plasmid

    #187106

    Purpose

    Expression of rHCaMP in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    rHCaMP
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJC20_cam1-T6C

    Plasmid

    #182427

    Purpose

    Bacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1 with the T6C residue replacement to allow labelling

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    cam1 (cam1 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    T6C substitution

    Promoter

    T7

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT5T_Rev7_1-211_SA112_KVK

    Plasmid

    #105634

    Purpose

    Expresses mouse monomeric Rev7 protein

    Depositor

    Thanos Halazonetis

    Insert

    Rev7 (Mad2l2 Mouse)
    

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcrVIA4

    Plasmid

    #164863

    Purpose

    Inducible expression of AcrVIA4 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    AcrVIA4
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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