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pJC25SUP35

Plasmid

#1096

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pET28b-MseLig1

Plasmid

#107071

Purpose

Expresses M. sedula DNA Ligase 1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

DNA ligase 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3F03

Plasmid

#70962

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Utx (Utx Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-TRPgamma

Plasmid

#83271

Purpose

Mammalian expression plasmid for Drosophila TRPgamma cDNA

Depositor

Craig Montell

Article

Xu et al Neuron. 2000 Jun;26(3):647-57.

Insert

TRPgamma (Trpgamma Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/PH* (W495L) uGFP

Plasmid

#14564

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

PH* (W495L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Smurf2 C716A

Plasmid

#13505

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C716A: catalytically inactive

Promoter

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pMX Vav1 Y3F/CR* (C529S) uGFP

Plasmid

#14560

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CR* (C529S)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2G08

Plasmid

#70915

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Parg (Parg Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2F12

Plasmid

#71060

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Chd3 (Chd3 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2D09

Plasmid

#71017

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED15 (MED15 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3D10

Plasmid

#71004

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED23 (MED23 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B02

Plasmid

#70782

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED9 (MED9 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pKM568

Plasmid

#134217

Purpose

Baculovirus expression of CENP-C

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

GST-CENP-C

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3F06

Plasmid

#70920

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc4 (Orc4 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/NSH3* (P651L) uGFP

Plasmid

#14562

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/NSH3* (P651L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/SH2* (R696K) uGFP

Plasmid

#14565

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/SH2* (R696K)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pJExpress414_GmPOPB-D661A

Plasmid

#92236

Purpose

Express D661A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-D661A mutant

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Aspartate 661 to Alanine

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/CSH3* (P833L) uGFP

Plasmid

#14566

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CSH3* (P833L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1C09

Plasmid

#70834

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MRG15 (MRG15 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1E08

Plasmid

#70829

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Cdk9 (Cdk9 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/Grb2bindb* (P657A) uGFP

Plasmid

#14563

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/Grb2bindb* (P657A)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G09

Plasmid

#70837

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIEalpha (TfIIEalpha Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4D02

Plasmid

#71023

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mof (mof Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1E04

Plasmid

#70800

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

asf1 (asf1 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2F08

Plasmid

#70914

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Ssrp (Ssrp Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
POSV801-SP44-SnaAinBCDEOT1T2

Plasmid

#225056

Purpose

Heterologous expression in Streptomyces with inactive SnaA

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaA

Use

Tags

Expression

Bacterial

Mutation

S556A/S1616A

Promoter

SP44

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEinOT1T2

Plasmid

#225057

Purpose

Heterologous expression in Streptomyces with inactive SnaE

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaE

Use

Tags

Expression

Bacterial

Mutation

deltaA46-V801

Promoter

SP44

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-T-Mce3R_K262A

Plasmid

#225728

Purpose

To express Mce3R mutant K262A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

K262A

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelG

Plasmid

#225729

Purpose

To express MelG (Rv1937) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-VapBC35

Plasmid

#225733

Purpose

To express VapBC35(Rv1962) Toxin antitoxin in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

vapBC35

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge