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pFloxin-IRES-Ofd1-Myc-A80T

Plasmid

#24575

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc-G139S

Plasmid

#24576

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Glycine 139 changed to serine (codon GGT to AGT)

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-Ofd1-Myc-S437R

Plasmid

#24577

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Serine 437 changed to Arginine (codon AGT to CGT)…

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pOX + JShal XR

Plasmid

#16148

Depositor

Larry Salkoff

Article

Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

Insert

Polyorchis penicillatus potassium channel gamma subunit (LOC721209, Ggamma1 jelly fish)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
Pprm1-luciferase; his-3

Plasmid

#137728

Purpose

Communication-activated luciferase in Neurospora crassa

Depositor

Louise Glass

Article

Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

Inserts

Luciferase
Pprm-1 Promoter

Use

Luciferase; Neurospora crassa expression

Mutation

Codon-optimized for N. crassa

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

His6 and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC Ad VAI

Plasmid

#175518

Purpose

For mammalian expression of adenovirus VAI RNA

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

Ad VAI RNA

Use

Adenoviral

Promoter

lac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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ORF57 Pr pGL4.16

Plasmid

#120378

Purpose

Firefly luciferase reporter with KSHV ORF57 early gene promoter

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF57 Promoter

Use

Luciferase

Promoter

ORF57 Promoter

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68

Plasmid

#162650

Purpose

Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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ORF31-2xStrep pCDNA4

Plasmid

#129744

Purpose

Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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ORF30-2xStrep pCDNA4

Plasmid

#129743

Purpose

Expresses KSHV ORF30 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF30 (ORF30 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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2xStrep-ORF34 pCDNA4

Plasmid

#120376

Purpose

Expresses KSHV ORF34 with Nterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF34 (ORF34 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18-3xFLAG pCDNA4

Plasmid

#120372

Purpose

Expresses KSHV ORF18 with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-BFLF1

Plasmid

#162661

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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ORF24-2xStrep pCDNA4

Plasmid

#129742

Purpose

Expresses KSHV ORF24 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF24 (ORF24 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 153-405-YFP

Plasmid

#18689

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 153-405

Tags

YFP

Expression

Yeast

Mutation

Prion domain of Rnq1

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP-Ofd1-Myc-S75F

Plasmid

#24580

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

serine 75 changed to phenylalanine (codon TCT cha…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-A80T

Plasmid

#24579

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge