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Plasmid#174661PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.DepositorInsertHfq
UseTagsalpha NTDExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
UseTagsMaltose binding protein and His tagExpressionBacterialMutationPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL_yEGFP_hc
Plasmid#98746PurposeReporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.DepositorInsertYeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromotersyntheticAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CTP-mCitrine
Plasmid#117989PurposeChloroplast-targeted expression of mCitrine controlled by the CaMV 35S promoterDepositorInsertCTP-mCitrine
UsePlant/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nup88 (551-741)
Plasmid#64252PurposeMammalian expression of C-terminal domain of Nup88DepositorInsertNup88 (NUP88 Human)
UseTagshis and mycExpressionMammalianMutationC-terminal domain (551-741)PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3b
Plasmid#166054Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertOrbicella faveolata caspase-3b
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3a
Plasmid#166053Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspaseDepositorInsertOrbicella faveolata caspase-3a
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp3
Plasmid#166052Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertPorites astreiodes caspase-3
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp7a
Plasmid#166051Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspaseDepositorInsertPorites astreiodes caspase-7a
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CTP-fluoA
Plasmid#117990PurposeChloroplast-targeted expression of CURT_fluoA controlled by the CaMV 35S promoterDepositorInsertCTP-CURT_fluoA
UseSynthetic Biology; Plant/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CTP-fluoB
Plasmid#117991PurposeChloroplast-targeted expression of CURT_fluoB controlled by the CaMV 35S promoterDepositorInsertCTP-CURT_fluoB
UseSynthetic Biology; Plant/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CTP-fluoE
Plasmid#117992PurposeChloroplast-targeted expression of CURT_fluoE controlled by the CaMV 35S promoterDepositorInsertCTP-CURT_fluoE
UseSynthetic Biology; Plant/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/CURT_flouB
Plasmid#117995PurposeExpression of CURT_flouB controlled by the CaMV 35S promoterDepositorInsertCURT_flouB
UseYeast/bacteria binary vectorTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-hLC3B-pcDNA3.1
Plasmid#40827DepositorInsertLC3B (MAP1LC3B Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP
Plasmid#138327Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1a
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1b-EGFP
Plasmid#138328Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1b
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a
Plasmid#205304PurposeExpression of WHaloCaMP1a in E. coliDepositorInsertWHaloCaMP1a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only