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Showing: 281 - 300 of 142055 results

  1. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid
    #183790
    Purpose
    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.
    Depositor
    Jay Shendure
    Article
    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-
    Inserts
    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    G>A in the gRNA scaffold- please see depositor…
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    183790-plasmid-map-sequence-id-374366

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  6. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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  7. sgRNA(MS2)_zeo insert c-MYC

    Plasmid
    #164636
    Purpose
    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
    Depositor
    Günter Schneider
    Insert
    target sequence for c-Myc activation (MYC Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164636-plasmid-map-sequence-id-326094

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  10. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid
    #124660
    Purpose
    reporter protein which emits bright blue fluorescence
    Depositor
    Yutaka Kuroda
    Article
    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1
    Insert
    Gaussia luciferase-C9D-TGinsert
    Use
    Tags
    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…
    Expression
    Bacterial
    Mutation
    changed glutamic acid 100 to alanine, and glycine…
    Promoter
    T7 promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    124660-plasmid-map-sequence-id-255641

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  11. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid
    #139770
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal TwinStrep tag
    Use
    Tags
    3C - TwinStrep tag
    Expression
    Mutation
    Promoter
    PH
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    139770-plasmid-map-sequence-id-324179

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Showing: 281 - 300 of 142055 results