We narrowed to 3,114 results for: GAI

1. pX330_gRNA_H19-DMR-2

   Plasmid

   #159930

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_gRNA_H19-DMR-1

   Plasmid

   #159929

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.2-Cx43-M100L-siResist

   Plasmid

   #49854

   Purpose

   Encodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Methionine100 mutated to Leucine. Contains severa…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

4. shp53 pLKO.1 puro

   Plasmid

   #19119

   Purpose

   3rd gen lentiviral vector for knocking down p53 gene expression

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against tumor protein p53 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

   Plasmid

   #64216

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. shCD44-2 pRRL

   Plasmid

   #19123

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against CD44 (CD44 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPRv2_RB1#1

   Plasmid

   #174150

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   RB1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB.DEST-ITGB1(WT)-mRuby2

   Plasmid

   #215448

   Purpose

   Piggybac expression vector with integrin beta1 tagged with mRuby2

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Transposon-based stable expression

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Silent mutations added to disrupt shRNA binding a…

   Promoter

   CAG

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

   Plasmid

   #220493

   Purpose

   To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. shRNA Caspase 1 (pSicoR mCherry)

    Plasmid

    #53575

    Purpose

    shRNA against human pro-caspase 1

    Depositor

    Warner Greene

    Article

    Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

    Insert

    shRNA Caspase 1 (CASP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; EF1a for mCherry

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

11. shp63alpha pLKO.1 puro

    Plasmid

    #19120

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against tumor protein p63 (TP63 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid

    #170185

    Purpose

    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Inserts

    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid

    #170184

    Purpose

    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Inserts

    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRC/CMV-TYK2V678F-VSV

    Plasmid

    #139349

    Purpose

    Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

    Depositor

    Sandra Pellegrini

    Article

    Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-V678F

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTER RB1shRNA human

    Plasmid

    #66886

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against RB1

    Depositor

    Maria Alvarado-Kristensson

    Article

    Ehlen et al J Biol Chem. 2012 May 18;287(21):17241-7

    Insert

    retinoblastoma 1 (RB1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pKHH030_sgRB1#2

    Plasmid

    #174145

    Purpose

    Lentiviral vector expressing a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CDK16_HUMAN_D0

    Plasmid

    #79696

    Purpose

    This plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDK16 (CDK16 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pET-pro-siRNA-V2-LMNA

    Plasmid

    #111088

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-myc-Sema4D-CD4-FLAG

    Plasmid

    #51601

    Purpose

    expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    

    Use

    Tags

    FLAG and myc

    Expression

    Mammalian

    Mutation

    Sema4D amino acids 660-861 were replaced with CD4…

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid

    #163359

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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