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pJH726_pJH_LldR_PlldR-sfGFP.15TAG

Plasmid

#163730

Purpose

Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.15TAG

Use

Tags

Expression

Bacterial

Mutation

gfp with first 15 leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH773_pTech_PlldR-HHRz-supP(opal)

Plasmid

#163732

Purpose

Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-ctrl

Plasmid

#85741

Purpose

shRNA ctrl

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA ctrl

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

Available since

April 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tipin

Plasmid

#22889

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
PcDNA3.1-Flag

Plasmid

#210342

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

Flag tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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Paxillin

Plasmid

#186142

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre-IRES2-GFP

Plasmid

#26646

Purpose

Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

Depositor

Anjen Chenn

Article

Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

Insert

Cre

Use

Cre/Lox

Tags

GFP and IRES2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-TCF1

Plasmid

#40620

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

TCF7 (TCF7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLG026 (tmn)

Plasmid

#157904

Purpose

Expresses the tmn defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

tmn (transmembrane ATPase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPL5606_TEF1*-Cre_ADE2

Plasmid

#60932

Purpose

Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 zeta

Plasmid

#116888

Purpose

Expresses HA-tagged human 14-3-3 zeta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 zeta (YWHAZ Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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ALKBH5-dCas9

Plasmid

#134783

Purpose

Expression fusion protein ALKBH5-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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phL1A-pcDNA3

Plasmid

#12307

Depositor

Vance Lemmon

Article

Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

Insert

L1CAM (L1CAM Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-30)

Plasmid

#199167

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(-30)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLG031 (upx)

Plasmid

#157909

Purpose

Expresses the upx defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

upx (putative PD-(D/E)xK family phosphodiesterase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3CD

Plasmid

#174190

Purpose

Expresses PIK3CD in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3CD (PIK3CD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-zGrad

Plasmid

#119716

Purpose

Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

Depositor

Holger Knaut

Article

Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

Insert

zGrad

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax WT

Plasmid

#23284

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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FTO-dCas9

Plasmid

#134781

Purpose

Expression fusion protein FTO-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

FTO and inactive dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Use

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHM6-alphasynuclein-WT

Plasmid

#40824

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Use

Tags

6x His and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pRDA_479

Plasmid

#179099

Purpose

BE Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e (SpG)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin4A (CUL4A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact EGFP

Plasmid

#187686

Purpose

In vivo visualization of actin

Depositor

Mary Beckerle

Article

Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDestattB (pCM268)

Plasmid

#68313

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

Depositor

Leonard Zon, Christian Mosimann

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLG010 (DRT type 2)

Plasmid

#157888

Purpose

Expresses the DRT type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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