We narrowed to 16,109 results for: form

1. Sema6a.z-Fc-His

   Plasmid

   #72164

   Purpose

   Expresses the extracellular region of the Sema6A, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6a.z (Sema6a Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Sema4f.a-AP-His

   Plasmid

   #72032

   Purpose

   Expresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema4f.a (Sema4f Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS

   Plasmid

   #121828

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7) (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

   Plasmid

   #121806

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + EF1a::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KH001: pMVP (L3-L2) V5 epitope tag + WPRE

   Plasmid

   #121762

   Purpose

   pMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   V5 epitope tag + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDK480

   Plasmid

   #134197

   Purpose

   Mammalian expression of Flip in SKAP and IRES GFP

   Depositor

   Iain Cheeseman

   Article

   Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

   Insert

   untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)
   

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Alternate Start, G5 siRNA hardened

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCellFree_G03 GTF2A1

   Plasmid

   #67076

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2A1 (GTF2A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLacIBD::SDRX (GB0673)

   Plasmid

   #68183

   Purpose

   Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   LacI Binding Domain fused to SRDX Transcriptional Repressor
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ntn5.z-Fc-His

   Plasmid

   #72109

   Purpose

   Expresses the entire Netrin 5, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntn5 (Ntn5 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ntn5.y-AP-His

    Plasmid

    #71982

    Purpose

    Expresses the entire Netrin 5, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn5.y (Ntn5 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Ntn5.z-AP-His

    Plasmid

    #71983

    Purpose

    Expresses the entire Netrin 5, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn5.z (Ntn5 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiGuide-Puro-CTRLg2 (CG510)

    Plasmid

    #139456

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMX-Flag-WIP 460 deletion

    Plasmid

    #11773

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP aa1-460 (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    aa461-503 deleted

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 merlin 308-579

    Plasmid

    #11626

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Carboxy-terminal common domain of merlin.

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA

    Plasmid

    #108844

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03 BTF3

    Plasmid

    #67059

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. p3E-Dnm2a-DN

    Plasmid

    #109572

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative Dynamin2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dynamin2a (dnm2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    dominant negative

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCellFree_G03 SMAD2

    Plasmid

    #67095

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Mouse c-myc (exon I)

    Plasmid

    #8446

    Depositor

    Frederic Mushinski

    Article

    Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

    Insert

    Mouse c-myc (exon I) (Myc Mouse)
    

    Expression

    Bacterial

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

20. Sema6d.5-AP-His

    Plasmid

    #72045

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.5 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. KZ701: pMVP (L3-L2) AID + polyA

    Plasmid

    #121791

    Purpose

    pMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to gene

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    AID + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KY801: pMVP (L3-L2) P2A-eGFP + polyA

    Plasmid

    #121764

    Purpose

    pMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCellFree_G03 TYK2

    Plasmid

    #67133

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TYK2 (TYK2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    A435S

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pSLIK-Venus-TmiR-G13

    Plasmid

    #25741

    Purpose

    Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LA102: pMVP (L3-L2) P2A-eYFP + WPRE

    Plasmid

    #121778

    Purpose

    pMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eYFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FB012

    Plasmid

    #119709

    Purpose

    Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC:hph:Ttub
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LA301: pMVP (L3-L2) P2A-Hygro + WPRE

    Plasmid

    #121787

    Purpose

    pMVP L3-L2 entry plasmid, contains Hygro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Hygro + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LA802: pMVP (L3-L2) AID + WPRE

    Plasmid

    #121794

    Purpose

    pMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    AID + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KO801: pMVP (L5-L4) Hygro-P2A

    Plasmid

    #121718

    Purpose

    pMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Hygro-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107322

    Purpose

    Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) is fused to SaCas9

    Promoter

    CMV IE93

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid

    #107325

    Purpose

    Expresses SpCas9 (R1335K) fused to NmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) is fused to NmCas9

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid

    #107326

    Purpose

    Expresses SpCas9 (R1333S) fused to NmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1333S) is fused to NmCas9

    Promoter

    CMV IE93

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Sema6d.4-Fc-His

    Plasmid

    #72170

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.4 (Sema6d Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. M26I DJ-1 TEV site pET15b

    Plasmid

    #60685

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26I DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26I and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

35. FgH1tUTG_huMcl-1.2

    Plasmid

    #85531

    Purpose

    Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Mcl-1.2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTH743-CEN-RLuc/slowstaCFLuc

    Plasmid

    #38223

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Unc5c.y-Fc-His

    Plasmid

    #72181

    Purpose

    Expresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.y (Unc5c Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Unc5c.y-AP-His

    Plasmid

    #72055

    Purpose

    Expresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.y (Unc5c Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Unc5d.a-AP-His

    Plasmid

    #72057

    Purpose

    Expresses the extracellular region of the Unc5D, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5d.a (Unc5d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Unc5a.z-AP-His

    Plasmid

    #72051

    Purpose

    Expresses the extracellular region of the Unc5A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5a.z (Unc5a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Unc5a.a-Fc-His

    Plasmid

    #72176

    Purpose

    Expresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5a.a (Unc5a Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Unc5d.a-Fc-His

    Plasmid

    #72183

    Purpose

    Expresses the extracellular region of the Unc5D, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5d.a (Unc5d Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCellFree_G03 BMPR2

    Plasmid

    #67127

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

44. RCAS (B) flag Lunatic Fringe (CT#471)

    Plasmid

    #13980

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Lunatic Fringe (LFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

45. pRVH1-hygro shStx3

    Plasmid

    #40071

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Stx3 shRNA-1
    

    Use

    Retroviral

    Promoter

    H1

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

46. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

47. Neo1.c-AP-His

    Plasmid

    #71965

    Purpose

    Expresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Neo1.c (Neo1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Unc5c.z-Fc-His

    Plasmid

    #72182

    Purpose

    Expresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.z (Unc5c Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Unc5d.z-Fc-His

    Plasmid

    #72185

    Purpose

    Expresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5d.z (Unc5d Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. RCAS (A) flag Lunatic Fringe (CT#479)

    Plasmid

    #13979

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Lunatic Fringe (LFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only