Purpose
Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
Inserts
dSpCas9-VPR
Bar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
Use
CRISPR and Synthetic Biology; Fungal genome integ…
Tags
VPR (VP64-p65-Rta) , NLS
Available Since
Sept. 11, 2020
Availability
Academic Institutions and Nonprofits only