Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,778 results for: LYC;

Showing: 2981 - 3000 of 3778 results

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pKG-GW1-hCIT

Plasmid

#203166

Purpose

Yeast expression vector for human DHDDS

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

DHDDS (DHDDS Human)

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM

Plasmid

#202415

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

Lacking DTHL

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-C-TEV-HAT

Plasmid

#202597

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL FBM

Plasmid

#202417

Purpose

Expression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Mutation

H477/R479/S481>AAA mutation

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA1

Plasmid

#201592

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA2

Plasmid

#201593

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-GPI_sgRNA2

Plasmid

#201595

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

GPI (GPI Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L3_sgRNA1

Plasmid

#201604

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L3_sgRNA2

Plasmid

#201605

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET-SU(Z)12(624-900)-H6

Plasmid

#183772

Purpose

Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

Insert

SU(Z)12 residues 624-900 (Su(z)12 Fly)

Tags

His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-C.37-AVI

Plasmid

#181702

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-SD1-C.37 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452Q, F490S

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-C.37-AVI

Plasmid

#181696

Purpose

Expresses SARS-CoV-2 NTD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-C.37 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G75V, T76I, R246N, ∆S247-∆D253,

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L452R-AVI

Plasmid

#176442

Purpose

Expresses SARS-CoV-2 RBD domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-L452R (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452R

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-S477N-AVI

Plasmid

#176443

Purpose

Expresses SARS-CoV-2 RBD domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-S477N (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

S477N

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-B.1.618-AVI

Plasmid

#176438

Purpose

Expresses SARS-CoV-2 NTD domain from the B.1.618 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-B.1.618 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Y145del, H146del

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-N501Y-AVI

Plasmid

#176439

Purpose

Expresses SARS-CoV-2 RBD domain containing N501Y mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-N501Y (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

N501Y

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-B.1.1.7-AVI

Plasmid

#176424

Purpose

Expresses SARS-CoV-2 NTD domain from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-B.1.1.7 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

69-70 del, 144del

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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