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pCfB3052(gRNA X-4, XI-3, XII-5)

Plasmid

#73294

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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shBIRC5 # 1

Plasmid

#42553

Depositor

William Hahn

Publication

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBIRC5 (BIRC5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR_zeo_sgTP53

Plasmid

#183180

Purpose

pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2cpV

Plasmid

#37471

Depositor

Amy Palmer, Roger Tsien

Publication

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D2cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Gal:mCherry-uTEV3

Plasmid

#135453

Purpose

Yeast expression of uTEV3 fused to mCherry

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-uTEv3

Tags

mCherry

Expression

Yeast

Mutation

S219V mutation improves stability , I138T/S153N/T…

Promoter

Gal

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Publication

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDA019

Plasmid

#131452

Purpose

control expression vector

Depositor

Kathleen Burns

Publication

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Type

Empty backbone

Expression

Mammalian

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMS73

Plasmid

#110629

Purpose

CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating

Depositor

Regine Kahmann

Publication

Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d

Inserts

short U6 promoter
cas9
tnos terminator

Use

CRISPR; Self-replicating in ustilago maydis, conf…

Tags

N-terminal NLS, C-terminal HA-tag +NLS

Expression

Bacterial

Mutation

Streptococcus pyogenes gene codon-optimized for U…

Promoter

U. maydis hsp70 promoter (Kronstad and Leong, 198…

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mTFP1-Vinculin-23

Plasmid

#55516

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Vinculin (VCL Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pORANGE GFP-RAB11a KI

Plasmid

#131499

Purpose

Endogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rab11a, Rab11a Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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MTCH2_pLX307

Plasmid

#98354

Purpose

Lentiviral expression of MTCH2

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Gfi1 NGFR

Plasmid

#44630

Depositor

Ellen Rothenberg

Publication

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Gfi1 (Gfi1 Mouse)

Use

Retroviral

Tags

IRES and truncated NGFR

Expression

Mammalian

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
ACE2(-252)-luc

Plasmid

#31111

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Publication

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -252 to +103

Available since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-gp78c/2767

Plasmid

#11429

Depositor

Allan Weissman

Publication

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78c (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-Flag-Tet3 CD Mut

Plasmid

#72222

Purpose

Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

Depositor

Yi Zhang

Publication

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET3 catalytic domain (cata…

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
BFP

Plasmid

#201383

Purpose

Control nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

BFP

Expression

Mammalian

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIMP1-Pthl

Plasmid

#90485

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Publication

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pccGFPKAN

Plasmid

#73649

Purpose

Fluorescent reporter for transmembrane protein dimerization.

Depositor

Alessandro Senes

Publication

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Type

Empty backbone

Expression

Bacterial

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Publication

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CAG promoter

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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