We narrowed to 8,294 results for: construct

1. pRSET FLIPglu-600uDelta13

   Plasmid

   #13559

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16A Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   F16A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. LI F-G Spec

   Plasmid

   #104521

   Purpose

   LI resistance gene module

   Depositor

   Thomas Lahaye

   Article

   Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

   Insert

   Spectinomycin resistance gene
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CH-GECO2.1

   Plasmid

   #52099

   Purpose

   mCherry-based Calcium indicators

   Depositor

   Robert Campbell

   Article

   Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

   Insert

   CH-GECO2.1
   

   Use

   Tags

   6 His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD promotor

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pJMD14

   Plasmid

   #138654

   Purpose

   phiOZJ integrating Streptomyces expression vector, intact RDF

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PermE* variant sequence from pDA1652

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOGG010

   Plasmid

   #113984

   Purpose

   Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   RK2 (IncP)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG81-pLas-tetO-deGFPssrA

   Plasmid

   #102454

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Use

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   pLas-tetO

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mUAVbla:tetR 5′

   Plasmid

   #149379

   Purpose

   mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Insert

   tetA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpic

   Plasmid

   #84372

   Purpose

   3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOGG011

   Plasmid

   #113985

   Purpose

   Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   pBBR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-TALE1

    Plasmid

    #27969

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4092694

    Plasmid

    #153907

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. p3E-P2A-CFP no-pA

    Plasmid

    #80827

    Purpose

    P2A followed by CFP without pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-P2A-CFP no pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

    Plasmid

    #199756

    Purpose

    Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAMP-CY1_NF1 T2 (+minintr, KDR)

    Plasmid

    #119201

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 2 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNH034

    Plasmid

    #42150

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    RT-cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV 1-5

    Plasmid

    #68411

    Purpose

    Lentiviral backbone for use with GMAP

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Type

    Empty backbone

    Use

    Lentiviral; Gmap

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOGG012

    Plasmid

    #113986

    Purpose

    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Partition genes (parABCDE)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK1200

    Plasmid

    #84219

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, EF1alpha promoter, mCherry

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFGC5941-PacI

    Plasmid

    #44020

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-H1-SGIPZ_NF1 sh1miR

    Plasmid

    #119206

    Purpose

    knockdown of NF1

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    sh1miR targeting NF1 (NF1 Rat, Mouse, Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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