We narrowed to 9,406 results for: Coli

1. pGEx-4T-1-YTHDF1

   Plasmid

   #70087

   Purpose

   Encode YTHDF1 with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET26b_AT118-L-V5-His

   Plasmid

   #222848

   Purpose

   E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOPINK-OTUD2 (full-length, aa 1-348)

   Plasmid

   #61409

   Purpose

   Expresses human OTUD2 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD2 (YOD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEx-4T-1-YTHDF3

   Plasmid

   #70088

   Purpose

   Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET-32a(+)-TGFB1m

   Plasmid

   #120286

   Purpose

   Produce human recombinant mTGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β1
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promotor

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNCST-AausFP1

   Plasmid

   #129499

   Purpose

   Expresses AausFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET30a-SARS-CoV2-NP

   Plasmid

   #165090

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pC001 - huLshC2C2-MBP for bacterial expression

   Plasmid

   #79150

   Purpose

   Expresses human codon-optimized LshC2c2 for purification in E. coli

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   C2c2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GST-PS-Grb2 (XSB363)

   Plasmid

   #78505

   Purpose

   Construct for expressing Grb2 in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   Grb2 (GRB2 Human)
   

   Use

   Tags

   GST followed by a prescission protease claevage s…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSFDuet-gst-xr

    Plasmid

    #214761

    Purpose

    Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

    Depositor

    Pimchai Chaiyen

    Article

    Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

    Insert

    glutathione S-transferases fused with xylose reductase
    

    Use

    Tags

    Glutathione S-transferases

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pH-ABE8e-spG

    Plasmid

    #200775

    Purpose

    For plant adenine base editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

    Insert

    nCas9-spG(D10A)-TadA8e
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

    Promoter

    maize Ubiquitin-1, OsU3

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

    Plasmid

    #113898

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF-G1TFAKRS

    Plasmid

    #177311

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOPINK-OTUB1 (full-length, aa 1-271)

    Plasmid

    #61420

    Purpose

    Expresses human OTUB1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

    Plasmid

    #159579

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSW002-Pc-TorA(sp)-mNeonGreen

    Plasmid

    #205020

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA-mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNCST-avGFP

    Plasmid

    #129508

    Purpose

    Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    avGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p11d-aRPA (MSW#712)

    Plasmid

    #208078

    Purpose

    Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ATG-3925 (CBG2)

    Plasmid

    #167839

    Purpose

    Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

    Depositor

    Lance Encell

    Article

    Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

    Insert

    Click Beetle Green 2
    

    Use

    Tags

    6-His

    Expression

    Bacterial and Mammalian

    Mutation

    Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

    Promoter

    CMV, T7

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hPU.1ΔN165-pET28b

    Plasmid

    #199796

    Purpose

    Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

    Depositor

    Gregory Poon

    Article

    Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

    Insert

    spi1 (SPI1 Human)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p11d-tRPA (MSW#111)

    Plasmid

    #208066

    Purpose

    expression of human Replication Protein A in E. coli

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Insert

    RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RPA32 has a point mutation Thr88(ACC) -> Ile(A…

    Promoter

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pet16b-Avi-ubiquitin_rbs_BirA

    Plasmid

    #134897

    Purpose

    Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Inserts

    Ubiquitin (UBB Human)
    BirA
    

    Use

    Tags

    10HIS-AviTag-PreScission(3C)protease

    Expression

    Bacterial

    Mutation

    Promoter

    RBS and T7

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSF-MjCouRS

    Plasmid

    #229066

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    Methanococcus jannaschii tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pF420

    Plasmid

    #123659

    Purpose

    Produces cofactor F420 in E. coli

    Depositor

    Colin Jackson

    Article

    Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

    Inserts

    CofD
    FbiB
    FbiD
    FbiC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_R0040

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOPINK-OTUD1 (full-length, aa 1-481)

    Plasmid

    #61405

    Purpose

    Expresses human OTUD1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-HLA-cMyc-EcopT1R1

    Plasmid

    #113962

    Purpose

    mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R1 (TAS1R1 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    truncate N-terminal 24 residues

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-BirA-P2A-mCherry

    Plasmid

    #74056

    Purpose

    retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    BirA biotin ligase
    

    Use

    Retroviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOPINK-Cezanne (OTU, aa 53-446)

    Plasmid

    #61581

    Purpose

    Expresses human Cezanne (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne (OTUD7B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-52 and aa 447-843.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGST2-GST-TEV-NDP52

    Plasmid

    #187828

    Purpose

    Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pRSET-WHaloCaMP1b-EGFP

    Plasmid

    #205305

    Purpose

    Expression of WHaloCaMP1b-EGFP fusion in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1b-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Plasmid

    #159580

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. WT USP7 TRAF

    Plasmid

    #227226

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 62-205

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. A3Ai-Cas9n-UGI-NLS

    Plasmid

    #109425

    Purpose

    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET30a-Spike-E

    Plasmid

    #165096

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pOPINK-OTUD6A (full-length, aa 1-288)

    Plasmid

    #61416

    Purpose

    Expresses human OTUD6A (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRSET-WHaloCaMP1a-EGFP

    Plasmid

    #205303

    Purpose

    Expression of WHaloCaMP1a-EGFP fusion in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJJGL002

    Plasmid

    #180606

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    PlmCasX
    

    Use

    CRISPR

    Tags

    10xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ScSpe1 pET-20b(+)

    Plasmid

    #117145

    Purpose

    Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    SPE1
    

    Use

    Tags

    Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. His10-CD3z-SNAPf-ybbR (XSB507)

    Plasmid

    #78508

    Purpose

    Construct for expressing CD3z-SNAPf-ybbR in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD3z (Cytoplasmic domain) (CD247 Human)
    

    Use

    Tags

    His10 and SNAPf-ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. plenti_DCLK1 G533N

    Plasmid

    #163627

    Purpose

    Expresses DCLK1 G533N in E. coli

    Depositor

    Kenneth Westover

    Article

    Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

    Insert

    DCLK1 (DCLK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    G33N

    Promoter

    sv40

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

    Plasmid

    #61406

    Purpose

    Expresses human OTUD1 (OTU+UIM domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

    Plasmid

    #159689

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. plenti_DCLK1 G511N

    Plasmid

    #163626

    Purpose

    Expresses DCLK1 G511N in E. coli

    Depositor

    Kenneth Westover

    Article

    Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

    Insert

    DCLK1 (DCLK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    G511N

    Promoter

    sv40

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pQlinkH-PTPIP51(36-235)

    Plasmid

    #170533

    Purpose

    Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHEN2 F8-2

    Plasmid

    #186033

    Purpose

    Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge