Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,586 results for: Coli

Clear keywords
Showing: 7501 - 7550 of 9586 results

  9. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184738
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184738-plasmid-map-sequence-id-361531

    Enlarge

  10. pHS1068

    Plasmid
    #205962
    Purpose
    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    DinG HNH proteins and CRISPR array
    Expression
    Bacterial
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available Since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    205962-plasmid-map-sequence-id-421621

    Enlarge

  11. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153442-plasmid-map-sequence-id-310821

    Enlarge

  16. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184739
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184739-plasmid-map-sequence-id-361530

    Enlarge

  18. pGG426_UV5

    Plasmid
    #165608
    Purpose
    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1135V, S1136R, D1332K and R1333G mutations in Sp…
    Promoter
    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165608-plasmid-map-sequence-id-330965

    Enlarge

  20. pET11-PC-H6

    Plasmid
    #183777
    Purpose
    Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.
    Depositor
    Peter Harte
    Article
    Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113
    Insert
    Polycomb (Pc Fly)
    Tags
    6 His tag at the C-terminus
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    183777-plasmid-map-sequence-id-360072

    Enlarge

  22. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184740
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184740-plasmid-map-sequence-id-361528

    Enlarge

  37. pUDP010

    Plasmid
    #101166
    Purpose
    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101166-plasmid-map-sequence-id-199274

    Enlarge

  39. pAIDA_TEV_fap2EC

    Plasmid
    #246866
    Purpose
    Expression of Fusobacterium nucleatum ATCC23726 Fap2 extracellular domain (42-3271) with histag and TEV site in fusion with E. coli autotransporter AIDA
    Depositor
    Daniel Roderer
    Article
    Schopf et al Nat Commun. 2025 Aug 29;16(1):8104. doi:
    Insert
    F. nucleatum ATCC23726 Fap2 (extracellular domain, res. 42–3271)
    Tags
    8x histidine, AIDA autotransporter, AIDA signal s…
    Expression
    Bacterial
    Available Since
    Nov. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    246866-plasmid-map-sequence-id-493107

    Enlarge

  42. pHS2096

    Plasmid
    #239835
    Purpose
    NovaIscB E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1 and swap o…
    Promoter
    T7
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239835-plasmid-map-sequence-id-479109

    Enlarge

  43. pdnCas3-CDA1

    Plasmid
    #229535
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229535-plasmid-map-sequence-id-459013

    Enlarge

  44. pdnCas3-APOBEC1

    Plasmid
    #229537
    Purpose
    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229537-plasmid-map-sequence-id-459005

    Enlarge

Showing: 7501 - 7550 of 9586 results