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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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NLuc-GID4

Plasmid

#200878

Purpose

Mammalian expression of human GID4 with N-terminal NanoLuciferase fusion

Depositor

Stefan Knapp

Article

Owens et al bioRxiv 2023.01.17.524225

Insert

NanoLuciferase-GID4 (C17orf39 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-Flag-IKBKE

Plasmid

#15292

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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SOX2-P2A-tagBFP-HDR

Plasmid

#163751

Purpose

HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

SOX2 (SOX2 Human)

Use

CRISPR and Synthetic Biology

Tags

P2A-tagBFP

Expression

Mutation

Does not contain start codon to avoid random inte…

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmMavs_V5

Plasmid

#160100

Purpose

Expresses murine Mavs in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Mavs (Mavs Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-xF2X-P2A-Puro

Plasmid

#110873

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS-2X(3.7)

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Neo-Flag-IKBKE

Plasmid

#15265

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-puro/RAF1-P261A

Plasmid

#131728

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P261A

Promoter

CMV

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-cRAF-W22

Plasmid

#15270

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CRAF (RAF1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C-terminal deletion

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
iMb2-Control-Mosaic (SO273)

Plasmid

#99750

Purpose

Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-HCN1 [N70/28R]

Plasmid

#114512

Purpose

Mammalian Expression Plasmid of anti-HCN1 (Rat). Derived from hybridoma N70/28.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-HCN1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Hcn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-CAAX-SV40

Plasmid

#117815

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQC MCS IRES Puro

Plasmid

#110343

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.

Depositor

Sandra Martha Gomes Dias

Article

Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-CAAX-B-actin

Plasmid

#117810

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_sgFLNB-SK2

Plasmid

#119873

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK2 (FLNB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_sgFLNB-SK4

Plasmid

#119874

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK4 (FLNB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRosa26-Acceptor (AG103)

Plasmid

#99632

Purpose

To insert the Tre-Tight or UAS ifgMosaic Donor vectors

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Neo-Myr-Flag-IKBKE

Plasmid

#15267

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pGTag-NLS-TagRFP-B-actin

Plasmid

#117806

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge