We narrowed to 29,740 results for: REP

1. pAAV-TRE-IEo

   Plasmid

   #201992

   Purpose

   AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

   Depositor

   Wei Xu

   Article

   Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

   Insert

   Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Tight TRE

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-GSDMD_C39A-Myc

   Plasmid

   #201167

   Purpose

   Expression of GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   C39A

   Promoter

   MESV

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMVQA

   Plasmid

   #197894

   Purpose

   pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

   Depositor

   Thomas Klimkait

   Article

   Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

   Insert

   HIV-1 3' end (HXB2: 9045 till poly-A(10)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGK EGFP-LC3B

   Plasmid

   #200427

   Purpose

   Mammalian expression of fluorescently-tagged marker for autophagic vesicles

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET32a-hNRG1a

   Plasmid

   #199235

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

   Insert

   Neuregulin (NRG1 Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-MYO10ΔF3

   Plasmid

   #194857

   Purpose

   Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

   Insert

   MYO10 ΔF3 (MYO10 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncated MYO10 construct. MYO10 amino acids 1-1…

   Promoter

   CMV

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAGGS-Flex/3'USS(103)-GFP

   Plasmid

   #197890

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAGGS-Flex/3'USS(553)-GFP

   Plasmid

   #197888

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAGGS-Flex/3'USS(285)-GFP

   Plasmid

   #197889

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-ZNF354C_mutant_M41K-L42K-E43K

    Plasmid

    #194185

    Purpose

    Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

    Insert

    ZNF354C (ZNF354C Human)
    

    Use

    Tags

    Myc-DDK

    Expression

    Mammalian

    Mutation

    KRAB domain mutant M41K/L42K/E43K

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TERTp124_Gluc_CCR5

    Plasmid

    #192271

    Purpose

    Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    Promoter Reporter Insert within donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    -124 C to T

    Promoter

    TERT promoter driving expression of gaussia

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TERTp146_Gluc_CCR5

    Plasmid

    #192272

    Purpose

    Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

    Depositor

    Shantanu Chowdhury

    Article

    Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

    Insert

    Promoter Reporter Insert within donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    -146 C to T

    Promoter

    TERT promoter driving expression of gaussia

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNHT7

    Plasmid

    #186753

    Purpose

    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning backbone

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid

    #184636

    Purpose

    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Gat1 gene with Arginine 69 changed to Lysine, to …

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2h70CRET(#325)

    Plasmid

    #184060

    Purpose

    cyclofen-inducible CRE activation in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transient transgenesis

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Hsp70-4

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-mCherry-SynaptoZip

    Plasmid

    #177318

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pN1mcherry-NKD2G2A

    Plasmid

    #174334

    Purpose

    encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    mutation of a.a 2 Gly to Ala

    Promoter

    CMV

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1mcherry-NKD2EFMUT

    Plasmid

    #174333

    Purpose

    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    NKD2132DFD134>AFA (substitutions of D132 and D…

    Promoter

    CMV

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FG11F-C-FLAG-hNKD2

    Plasmid

    #174330

    Purpose

    encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MO70-C-FLAG-hNKD2

    Plasmid

    #174327

    Purpose

    encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Retroviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA4TO-3XFLAG Halo R99A nsp1

    Plasmid

    #175422

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R99A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAG-Bmpr2 gRNA resistant

    Plasmid

    #163408

    Purpose

    Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    gRNA-targeting sequences are substituted with nuc…

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEGFP_A893E-talin1 (1-2541)

    Plasmid

    #166116

    Purpose

    For expression of full-length mouse talin-1 carrying mutation A893E in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    A893E-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A893E

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP_L1539P-talin1 (1-2541)

    Plasmid

    #166119

    Purpose

    For expression of full-length mouse talin-1 carrying mutation L1539P in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    L1539P-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L1539P

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP_L2509P-talin1 (1-2541)

    Plasmid

    #166123

    Purpose

    For expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    L2509P-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L2509P

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP_S1750F-talin1 (1-2541)

    Plasmid

    #166120

    Purpose

    For expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    S1750F-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1750F

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEGFP_D2086V-talin1 (1-2541)

    Plasmid

    #166122

    Purpose

    For expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    D2086V-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D2086V

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGFP_R1368W-talin1 (1-2541)

    Plasmid

    #166117

    Purpose

    For expression of full-length mouse talin-1 carrying mutation R1368W in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    R1368W-talin1-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R1368W

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP_Y1389C-talin1 (1-2541)

    Plasmid

    #166118

    Purpose

    For expression of full-length mouse talin-1 carrying mutation Y1389C in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    Y1389C-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y1389C

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCAG-EGxxFP-PIGA

    Plasmid

    #153958

    Purpose

    Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    PIGA (a 401-bp fragment from intron 5 and exon 6)
    

    Use

    A plasmid specific for egxxfp assay

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    CAG promoter

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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