We narrowed to 22,191 results for: his

1. pFastBac Dual wt p110α/p85α

   Plasmid

   #199302

   Purpose

   co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

   Depositor

   Peter Vogt

   Article

   Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

   Insert

   His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
   

   Use

   Tags

   Avi and His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-beta-3

   Plasmid

   #27289

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin beta 3 (ITGB3 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSP1D1

   Plasmid

   #20061

   Purpose

   Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   deletion mutant (1-11) of MSP1; His-tag on N-term…

   Promoter

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBad-CA TAG126

   Plasmid

   #105836

   Purpose

   Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Use

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 126

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Use

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBudORF1-CH

   Plasmid

   #51290

   Purpose

   expresses the codon optimized ORF1 of L1RP with his tag

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

   Insert

   L1 ORF1 (L1RE1 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. (+)-Limonene Synthase

   Plasmid

   #112230

   Purpose

   (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

   Depositor

   Daniel Oprian

   Article

   Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

   Insert

   (+)-Limonene Synthase
   

   Use

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-52

   Promoter

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Ursula Jakob, Michael Gray

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-hDcp2

   Plasmid

   #72214

   Purpose

   bacterial expression of His-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEF6-myc-TBC1D15

    Plasmid

    #79148

    Purpose

    mammalian expression of TBC1D15

    Depositor

    Aimee Edinger

    Article

    Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

    Insert

    TBC1D15 (Tbc1d15 Mouse)
    

    Use

    Tags

    His, Myc, and V5

    Expression

    Mammalian

    Mutation

    S616N (please see depositor comment below)

    Promoter

    EF-1alpha

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HLTV-hTRIM21

    Plasmid

    #104973

    Purpose

    Purification of His-Lipoyl-hTRIM21 protein from bacteria

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Use

    Tags

    His-Lipoyl

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. Plasmid L5 attB::Pleft* mWasabi

    Plasmid

    #169409

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mWasabi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Plasmid L5 attB::Pleft* mScarlet

    Plasmid

    #169410

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mScarlet proper
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJPT002-M-HaloTag-1x FLAG

    Plasmid

    #202493

    Purpose

    Express "M" HaloTag

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    HaloTag
    

    Use

    Tags

    1x FLAG and Myristoylation site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMAL-c5x_UnaG

    Plasmid

    #163125

    Purpose

    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    UnaG
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FnR8-10

    Plasmid

    #182569

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCN-mBFP

    Plasmid

    #63847

    Purpose

    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Insert

    mBFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter and RBS was swapped. His-tag was removed…

    Promoter

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBad-mCherry-XL (R125K)

    Plasmid

    #188054

    Purpose

    Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    araBAD

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET30-RT1306

    Plasmid

    #131521

    Purpose

    Expresses engineered RT-1306 with His-tag

    Depositor

    Bryan Dickinson

    Article

    Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

    Insert

    RT-1306
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 myc TSC1

    Plasmid

    #12133

    Depositor

    Cheryl Walker

    Article

    Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FnR9-10

    Plasmid

    #182567

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBEL1284

    Plasmid

    #175960

    Purpose

    For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Inserts

    LetA
    LetB
    

    Use

    Tags

    6xHis 2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3 V5 hHR23A

    Plasmid

    #13053

    Depositor

    Steven Grossman

    Article

    Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

    Insert

    hHR23A (RAD23A Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    R325K and L355P relative to NP_001257291.1

    Promoter

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NLSx3-CRY2PHR-p65

    Plasmid

    #64124

    Purpose

    Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    CRY2PHR-p65
    

    Use

    Tags

    His, NLSx3, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJPT003-PM-HaloTag-1x FLAG

    Plasmid

    #202494

    Purpose

    Express "PM" HaloTag

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    HaloTag
    

    Use

    Tags

    1x FLAG and Palmitoylation-Myristoylation Site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Plasmid L5 attB:Psmyc double green

    Plasmid

    #169412

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mWasabi
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Plasmid L5 attB:Psmyc triple red

    Plasmid

    #169413

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet proper
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPD128 AND Gate, 3 repeats

    Plasmid

    #138937

    Purpose

    EYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    Logic 37 AND 158 NOT 43 3 repeats
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Logic 37 AND 158 NOT 43 3 repeats

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RNF146-c005

    Plasmid

    #173091

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    RNF146 (RNF146 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    R99-L183, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GDAP2-c005

    Plasmid

    #173110

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GDAP2 (GDAP2 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    M1-N231

    Promoter

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pWaldo-GFPe-SH1446

    Plasmid

    #112507

    Purpose

    Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

    Depositor

    Simon Newstead

    Article

    Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

    Insert

    POT family transporter
    

    Use

    Tags

    C terminal TEV GFP octa-His affinity tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFlag-CMV2-WBP2

    Plasmid

    #27478

    Depositor

    Marius Sudol

    Article

    Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

    Insert

    WW domain binding protein-2 (WBP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only