We narrowed to 12,111 results for: LEA

Showing: 3021 - 3040 of 12111 results

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HisMBP CFlm25

Plasmid

#78458

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie

Publication

Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

Insert

Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)

Tags

His and MBP

Expression

Bacterial

Promoter

tac

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5R18-CtermQ7

Plasmid

#51443

Purpose

Expresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domain

Depositor

David Liu

Publication

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Q7 C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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ND4.2-Left TALE-G1397-N-DddA11

Plasmid

#179681

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Publication

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR

Expression

Mammalian

Mutation

S1330I A1341V N1342S E1370K T1380I

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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hP2X3-cALFA

Plasmid

#160499

Purpose

For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

human P2X3 (P2RX3 Synthetic, Human)

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

ΔN5ΔC33-T13P/S15V/V16I

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

Plasmid

#134865

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Membrin (GOSR2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-cPHx2

Plasmid

#116854

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Publication

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem dimer)

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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RINS1(mut)

Plasmid

#107291

Purpose

Plasmid for the expression of the inactive mutant of RINS1 sensor

Depositor

Dmytro Yushchenko

Publication

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Insert

RINS1(mut)

Tags

sfGFP

Expression

Mammalian

Mutation

two Arg/Ser mutations at the beginning and in the…

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Publication

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42 (CDC42 Human)

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOTTC414 - pAAV EF1a hPREP

Plasmid

#59967

Purpose

An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Publication

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FLAG-Pol-II CC

Plasmid

#35180

Depositor

Benjamin Blencowe

Publication

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains C-terminal domain repeats (1-22)X2

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-PRKAA1ΔC

Plasmid

#30306

Depositor

Jay Brenman

Publication

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 ΔC (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids Pro 526 to Gln 548

Available since

Aug. 26, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Publication

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUB-nSypHer (NLS:sypHer:NLS)

Plasmid

#84731

Purpose

Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

nSypHer

Tags

NLS (nuclear localisation signal and NLS (nuclear…

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Cas7-11_crRNA

Plasmid

#197612

Purpose

For protein purification of Cas7-11

Depositor

Feng Zhang

Publication

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Cas7-11 with crRNA

Expression

Bacterial

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-AKAR4

Plasmid

#181957

Purpose

Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

Depositor

Jin Zhang

Publication

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-AKAR4 (AKAP5 Human)

Tags

AKAP79, Cerulean, HIS tag, and cpVenus(E172)

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBK092

Plasmid

#183146

Purpose

pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

Depositor

Daan Swarts

Publication

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EC2_3_dCas9_Mxi1_sgRNA

Plasmid

#163708

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
Mxi1+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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DiLiCre 2.0

Plasmid

#198752

Purpose

A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

Depositor

Jacco van Rheenen

Publication

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

Cre-PhoCI-ERT2-ERT2

Use

Lentiviral

Tags

Cre, kept in a photocleavable site, followed by 2…

Expression

Bacterial and Mammalian

Promoter

TRE3GV

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

Plasmid

#187580

Purpose

Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Publication

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NONO (NONO Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP8-Myc-FLAG (G418)

Plasmid

#86850

Purpose

mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Publication

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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