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pJH575

Plasmid

#172159

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop3); AraC

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH576

Plasmid

#172160

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 34B_sub-pop4); AraC

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K, E251D) PylD(A252V)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH19

Plasmid

#172141

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH60

Plasmid

#172142

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.CD

Expression

Bacterial

Mutation

PylCD partial operon (PylB deleted to assess impa…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH77

Plasmid

#172145

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*, P83*, Y184*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH123

Plasmid

#172146

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.B.(SD4)CD

Expression

Bacterial

Mutation

PylBCD operon (RBS inserted for PylC)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH126

Plasmid

#172147

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.SUMO-B.(SD4).CD

Expression

Bacterial

Mutation

PylBCD operon (SUMO tag inserted for PylB; RBS in…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#2

Plasmid

#163395

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#1

Plasmid

#163394

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#3

Plasmid

#163396

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBL-2

Plasmid

#162769

Purpose

Minimal backbone with AmpR

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Synthetic Biology; Molecular cloning

Promoter

no

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM862

Plasmid

#169085

Purpose

MoClo level 0 BvDODA1 coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvDODA1

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM1021

Plasmid

#169084

Purpose

MoClo level 0 BvCYP76AD1 coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvCYP76AD1

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 WD

Plasmid

#163580

Purpose

express Dcp2 ΔH1 WD at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

W50A, D54A, L255A

Promoter

DCP2 promoter

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1

Plasmid

#163576

Purpose

express Dcp2 ΔH1 (L255A) at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Lysine at position 255 is mutated to Alanine

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A-GFP

Plasmid

#166837

Purpose

Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

G2A

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pVG97

Plasmid

#164849

Purpose

Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA

Expression

Yeast

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300

Plasmid

#163577

Purpose

express Dcp2 300 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domian (301-970)

Promoter

DCP2 promoter

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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