We narrowed to 10,360 results for: plasmids 101

1. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86

   Plasmid

   #212538

   Purpose

   Encodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

   Plasmid

   #170201

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV51

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

   Plasmid

   #122982

   Purpose

   AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

   Depositor

   Matthew Kennedy

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   mCh-IRES-BoNT/B(1-146)-iLID
   

   Use

   AAV

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   iLID contains long lived V416I mutation

   Promoter

   human synapsin

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-noSV40-humanEC1.45_p300peak1

   Plasmid

   #173947

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   p300 peak 1 from SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-noSV40-mouseEC1.45

   Plasmid

   #173972

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Mouse orthologous SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. R620-M21-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.429

   Plasmid

   #170200

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.429 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.429 (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   S13I, W152C, L452R, D614G

   Promoter

   CMV51

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn INTRON HaloTag-STIM1 WPRE

   Plasmid

   #236233

   Purpose

   AAV expression of mouse STIM1 internally tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   HaloTag-Stim1 (Stim1 Mouse)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV hSyn INTRON mEmerald-STIM1 WPRE

   Plasmid

   #236234

   Purpose

   AAV expression of mouse STIM1 internally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mEmerald-Stim1 (Stim1 Mouse)
   

   Use

   AAV

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MKC84_HBB(HBA1reg-HBA1)

    Plasmid

    #232410

    Purpose

    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

    Depositor

    Kyle Cromer

    Article

    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. MKC83_HBB(HBBreg-HBA1)

    Plasmid

    #232409

    Purpose

    AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

    Depositor

    Kyle Cromer

    Article

    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)

    Plasmid

    #212402

    Purpose

    Encodes SARS-CoV-2 spike L5F,D614G,Q677H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike L5F,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L5F,D614G,Q677H

    Promoter

    CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

    Plasmid

    #212542

    Purpose

    Encodes SARS-CoV-2 Spike F456L for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F456L

    Promoter

    CAG

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-SARS-CoV-2 Spike-deltaC18 FL.1.5.1

    Plasmid

    #212539

    Purpose

    Encodes SARS-CoV-2 variant FL1.5.1 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike FL.1.5.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Spike-deltaC18 G75V (GTA) T76I (ATT)

    Plasmid

    #212443

    Purpose

    Encodes SARS-CoV-2 spike G75V, T76I for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike G75V, T76I (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G75V, T76I

    Promoter

    CAG

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTwist-SARS-CoV-2 Spike-deltaC18 del E484

    Plasmid

    #212564

    Purpose

    Encodes SARS-CoV-2 spike E484 deletion for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike E484 deletion (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E484 deletion

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1 T478R

    Plasmid

    #212534

    Purpose

    Encodes SARS-CoV-2 variant XBB.1 Spike T478R for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike XBB.1 T478R (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTwist-SARS-CoV-2 Spike-deltaC18 FD.1.1

    Plasmid

    #212537

    Purpose

    Encodes SARS-CoV-2 variant FD.1.1 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike FD.1.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.12.1

    Plasmid

    #212503

    Purpose

    Encodes SARS-CoV-2 variant BA.2.12.1 Spike for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike BA.2.12.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge