We narrowed to 8,158 results for: Dos;

1. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

   Plasmid

   #121944

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR SFFVp PHAkt Cerulean

   Plasmid

   #50837

   Purpose

   Fluorescent reporter for membrane PIP3 levels

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PHAkt (AKT1 )
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Cerulean

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P-1-eIF4E K119A

   Plasmid

   #199440

   Purpose

   Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

   Depositor

   Shin-ichi Hoshino

   Article

   Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

   Insert

   1977 (EIF4E Human)
   

   Use

   Tags

   GST-fusion protein

   Expression

   Bacterial

   Mutation

   changed lysine 119 to alanine

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS_L_EBOV

   Plasmid

   #103052

   Purpose

   Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   L gene of Ebola virus (L Zaire ebolavirus, Mayinga)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOO2-GW

   Plasmid

   #135097

   Purpose

   Gateway vector for expression of proteins in Xenopus oocytes

   Depositor

   Wolf Frommer

   Article

   Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

   Type

   Empty backbone

   Use

   Bacterial expression vector for generating sp6 tr…

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTF102

   Plasmid

   #134774

   Purpose

   A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.

   Depositor

   Kan Wang

   Article

   Frame et al Plant Physiol. 2002 May;129(1):13-22. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. eSrtA(4S-9) pET29b

   Plasmid

   #75146

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N98D S102C A104V A118T F122A K134R F144L I182V E1…

   Promoter

   T7

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 GFP-h-PKD2

   Plasmid

   #83451

   Purpose

   GFP-tagged PC2 for Eukaryotic expression (883)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-2 (PKD2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR V5-RNF14 C417A_IRES-mCherry

   Plasmid

   #198385

   Purpose

   RNF14 C417A expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RNF14 (RNF14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   catalytic cysteine mutation (C417A)

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP

    Plasmid

    #111518

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    calmodulin [Xenopus laevis] (calm2 Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D20A/D56AD93A/D129A

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-EN479

    Plasmid

    #86234

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse rosa26 locus
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MD-2

    Plasmid

    #20864

    Depositor

    Jie-Oh Lee

    Article

    Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

    Insert

    Myeloid differentiation factor 2 (LY96 Human)
    

    Use

    Tags

    protein A tag

    Expression

    Insect

    Mutation

    contains amino acids 19–160

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. 2xCBX7CD-IFP2.0C

    Plasmid

    #215754

    Purpose

    Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xCBX7CD-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS_VP35_EBOV

    Plasmid

    #103050

    Purpose

    Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. HsGRK6-mVenus

    Plasmid

    #137780

    Purpose

    Visualization of GRK6

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human GRK6 (GRK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH1371

    Plasmid

    #199631

    Purpose

    Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GPI
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAGGS_3E5E_luciferase

    Plasmid

    #103055

    Purpose

    Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    Ebolavirus minigenome
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS_VP30_EBOV

    Plasmid

    #103051

    Purpose

    Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR V5-RNF25 dRING_IRES-mCherry

    Plasmid

    #198387

    Purpose

    RNF25 (?RING) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RNF25 (RNF25 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    RING deletion (135-201)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPEPY-PF6-lacI

    Plasmid

    #85589

    Purpose

    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PF6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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