We narrowed to 17,089 results for: EMB

1. RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

   Plasmid

   #192234

   Purpose

   lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RPS4X sgRNA-2 lentiCRISPR v2 plasmid

   Plasmid

   #192232

   Purpose

   lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. RPS4X sgRNA-1 lentiCRISPR v2 plasmid

   Plasmid

   #192231

   Purpose

   lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC

   Plasmid

   #148442

   Purpose

   Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)
   

   Expression

   Mammalian

   Mutation

   1 silent mutation compared to the sequence of iso…

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

   Plasmid

   #178857

   Purpose

   Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

   Plasmid

   #178858

   Purpose

   Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET-62-SFFV-T2A-PuroR-polyA-R11b

   Plasmid

   #179893

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJET-64-SFFV-T2A-BlaR-polyA-R11b

   Plasmid

   #179895

   Purpose

   Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Expression

   Bacterial and Mammalian

   Promoter

   SFFV

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneo-lambdaN-HA-HsTNRC6A_1242-1709-F1359A_K

   Plasmid

   #146742

   Purpose

   Mammalian Expression of HsTNRC6A_1242-1709-F1359A

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   HsTNRC6A_1242-1709-F1359A (TNRC6A Human)
   

   Expression

   Mammalian

   Mutation

   five non silent mutations D1289G, G1515A, S1516G,…

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRESneo2 mycBioID-Tollip M240A,F241A

    Plasmid

    #178055

    Purpose

    myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Tags

    mycBioID

    Expression

    Mammalian

    Mutation

    Changed Methionine 240 to Alanine and changed Phe…

    Promoter

    CMV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUS_LC_charge_RtoK

    Plasmid

    #139127

    Purpose

    express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Expression

    Bacterial

    Mutation

    Add K and D to FUS LC so it has the same charge p…

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FB026

    Plasmid

    #119711

    Purpose

    Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA:YFP coding sequence:Terminator trpC
    

    Use

    Synthetic Biology

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-TEV-mCardinal

    Plasmid

    #105798

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCardinal

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-TEV-mCerulean

    Plasmid

    #105790

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCerulean

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-mCardinal-TEV

    Plasmid

    #105781

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCardinal-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-mCerulean-TEV

    Plasmid

    #105773

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCerulean-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. KLHL31 pgk (1088)

    Plasmid

    #62031

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    KLHL31 (Klhl31 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid

    #178057

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-FLAG
    

    Use

    AAV

    Tags

    FLAG-MBP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-MBP-TEV

    Plasmid

    #105771

    Purpose

    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    MBP-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N3/hArf6(WT)-EGFP

    Plasmid

    #79423

    Purpose

    Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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