We narrowed to 68,463 results for: nin

1. pUAS-Myc-BirA-G3-ER

   Plasmid

   #173815

   Purpose

   Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

   Depositor

   Norbert Perrimon

   Article

   Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

   Insert

   Myc-BirA-G3-ER
   

   Tags

   Myc

   Expression

   Insect

   Promoter

   UAS

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC-SUR-YR

   Plasmid

   #61768

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Sur gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Promoter

   Magnaporthe oryzae ILV promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. fck-Jaws-GFP-ER2

   Plasmid

   #65013

   Purpose

   Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-GFP-ER2
   

   Use

   Lentiviral

   Tags

   ER2 and GFP

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CaMKII

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. HTR1D-DuET

   Plasmid

   #213318

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HTR1D (HTR1D Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HTR1B-DuET

   Plasmid

   #213317

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HTR1B (HTR1B Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. fck-Jaws-KGC-GFP-ER2

   Plasmid

   #65012

   Purpose

   Lentivirus-mediated expression of Jaws under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   Lentiviral

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CaMKII

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. ADRB2-DuET

   Plasmid

   #213181

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRB2 (ADRB2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ADRA2A-DuET

   Plasmid

   #213177

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRA2A (ADRA2A Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-dGFP-JARID1C A388P

    Plasmid

    #74778

    Purpose

    retroviral expression of JARID1C A388P

    Depositor

    Giovanni Tonon

    Article

    Rondinelli et al J Clin Invest. 2015 Dec;125(12):4625-37.

    Insert

    JARID1C (KDM5C Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A388P

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pME mKate2-dynactin1-811

    Plasmid

    #105971

    Purpose

    entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    dynactin1-811 (DCTN1 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    contains only amino acids 1-811

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO/myc-His-A-YAP2-1st&2nd WW mut

    Plasmid

    #19061

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine,and Proline 202…

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant

    Plasmid

    #19000

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine, and Proline 2…

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pET-28a(+) PCMT1 H58 A214

    Plasmid

    #246496

    Purpose

    Recombinant protein expression of PCMT1 H58 A214

    Depositor

    Steven Clarke

    Article

    Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

    Insert

    Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Threonine 58 mutated to Histidine and Valine 214 …

    Promoter

    T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-FRET_PST-C

    Plasmid

    #228570

    Purpose

    Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Pancreastatin

    Depositor

    Geert van den Bogaart

    Article

    Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

    Insert

    Chromogranin-A (CHGA Human)
    

    Tags

    mCitrine and mScarlet-I

    Expression

    Mammalian

    Mutation

    FRET sensor; see explanatory note

    Promoter

    cmv

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-FRET_PST-N

    Plasmid

    #228571

    Purpose

    Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Pancreastatin

    Depositor

    Geert van den Bogaart

    Article

    Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

    Insert

    Chromogranin-A (CHGA Human)
    

    Tags

    mCitrine and mScarlet-I

    Expression

    Mammalian

    Mutation

    FRET sensor; see explanatory note

    Promoter

    cmv

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-FRET_CST-C

    Plasmid

    #228572

    Purpose

    Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Catestatin

    Depositor

    Geert van den Bogaart

    Article

    Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

    Insert

    Chromogranin-A (CHGA Human)
    

    Tags

    mCitrine and mScarlet-I

    Expression

    Mammalian

    Mutation

    FRET sensor; see explanatory note

    Promoter

    cmv

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-FRET_CST-N

    Plasmid

    #228573

    Purpose

    Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Catestatin

    Depositor

    Geert van den Bogaart

    Article

    Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

    Insert

    Chromogranin-A (CHGA Human)
    

    Tags

    mCitrine and mScarlet-I

    Expression

    Mammalian

    Mutation

    FRET sensor; see explanatory note

    Promoter

    cmv

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-mEGFP-hCTNNA1_P-linker 4E

    Plasmid

    #234564

    Purpose

    CTNNA1 Phospholinker phospho-mimic

    Depositor

    Cara Gottardi

    Article

    Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

    Insert

    mEGFP:hCTNNA1_P-linker 4E (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Mutation

    S641E, S652E, S655E, T658E

    Promoter

    CMV

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-mEGFP-hCTNNA1_P-linker 4A

    Plasmid

    #234563

    Purpose

    CTNNA1 Phospholinker phospho-mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al J Cell Biol. 2025 Mar 3;224(3):e20230812

    Insert

    mEGFP:hCTNNA1_P-linker 4A (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Mutation

    S641A, S652A, S655A, T658A

    Promoter

    CMV

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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