We narrowed to 31,032 results for: uros

1. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

   Plasmid

   #211463

   Purpose

   Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI-dead
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   TRE

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-AU1

   Plasmid

   #162102

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-FLAG

   Plasmid

   #162104

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-FLAG

   Plasmid

   #162110

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-AU1

   Plasmid

   #162085

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEpicVector3G_Puro-T2A-EGFP-VSVg-FLAG-AU1

   Plasmid

   #162088

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-FLAG-AU1

   Plasmid

   #162094

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-FLAG

   Plasmid

   #162081

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-StrepTagII-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

   Plasmid

   #133025

   Purpose

   Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Monoamine oxidase type A (MAOA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-488

   Promoter

   Human elongation factor 1 alpha

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-IRES-Puro GFP-3xFLAG

    Plasmid

    #201636

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. untargeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45215

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Aurora B FRET sensor
    

    Use

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-Pan-Neurofascin (extracellular) [A12/18R]

    Plasmid

    #177438

    Purpose

    Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid

    #89993

    Purpose

    Donor template for generation of SOX9-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-VQR-P2A-GFP-PGK-Puro

    Plasmid

    #110861

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid

    #122183

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9 (NEWENTRY )
    mNeonGreen
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52380

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -1620 - -1011 upstream from OCT4 ATG

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-HF1RA-P2A-GFP-PGK-Puro

    Plasmid

    #110866

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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