We narrowed to 22,372 results for: Sis

1. pKanB alpha

   Plasmid

   #223524

   Purpose

   Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

   Depositor

   Geoff Lin-Cereghino

   Article

   Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Sept. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCDB179-LCC

   Plasmid

   #218686

   Purpose

   Encodes codon optimized LCC enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   LCC
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF-CjPglB-LpxE

   Plasmid

   #128389

   Purpose

   Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Inserts

   PglB oligosaccharylktransferase
   LpxE phosphatase
   

   Use

   Tags

   Flag tag

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and pBAD

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 5'-myr-dEGFP-3'

   Plasmid

   #16075

   Depositor

   Erin Schuman

   Article

   Aakalu et al Neuron. 2001 May . 30(2):489-502.

   Insert

   Protein synthesis reporter (Camk2a Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Xenopus APC-myc

   Plasmid

   #16686

   Depositor

   Barry Gumbiner

   Article

   Vleminckx et al J Cell Biol. 1997 Jan 27. 136(2):411-20.

   Insert

   APC (apc.L Frog)
   

   Use

   Xenopus expression

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

8. Plasmid L5 attB::Pleft* mCherry

   Plasmid

   #169411

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mCherry
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHEE901

   Plasmid

   #91707

   Purpose

   CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWZLblasti-CTR1

    Plasmid

    #53157

    Purpose

    Expresses human CTR1 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    CTR1 (SLC31A1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pME-NR2F2

    Plasmid

    #138359

    Purpose

    Gateway compatible middle entry clone contains human full length NFR2F2 gene with stop codon

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    Human NR2F2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGE1109

    Plasmid

    #202773

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSP64-GFP-ePDZ-CAAX

    Plasmid

    #135603

    Purpose

    Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    Lifeact-GFP-ePDZ-CAAX
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    CAAX, EGFP, and ePDZ

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Plasmid L5 attB:Psmyc double green

    Plasmid

    #169412

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mWasabi
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Plasmid L5 attB:Psmyc triple red

    Plasmid

    #169413

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet proper
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK

    Plasmid

    #62670

    Purpose

    Vector for PhiC31o mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-PM

    Plasmid

    #197386

    Purpose

    To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    PDX1-T2A-MAFA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKM808

    Plasmid

    #134181

    Purpose

    Lentiviral sgRNA plasmid with blasticidin resistance

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDSAR-Cre-F2A-I-SceI

    Plasmid

    #62302

    Purpose

    Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    Cre recombinase, I-SceI nuclease
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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