We narrowed to 8,324 results for: gnal

1. pcDNA3-PM-CaNAR2

   Plasmid

   #64730

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   Lyn-CaNAR2
   

   Use

   Tags

   Cerulean3, N-terminal targeting sequence from Lyn…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE-tight2-Tmem98-FLAG

   Plasmid

   #124506

   Purpose

   Expresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)

   Depositor

   Renee van Amerongen

   Article

   van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

   Insert

   Tmem98 (Tmem98 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   tetO-CMV

   Available Since

   May 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

   Plasmid

   #213116

   Purpose

   Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   FRB-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. anti-Notch2_B6-pBIOCAM5

   Plasmid

   #39347

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
   

   Use

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. 10XSTAT92E–luciferase

   Plasmid

   #37393

   Depositor

   Norbert Perrimon

   Article

   Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

   Insert

   SOCS36E enhancer fragment (Socs36E Fly)
   

   Use

   Luciferase; Jak/stat reporter construct

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Hsp70

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV CAGG eGFP

   Plasmid

   #107707

   Purpose

   express eGFP in variety of cell types

   Depositor

   Troy Margrie

   Article

   Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xDBE_RE::Renilla

   Plasmid

   #124535

   Purpose

   Foxo Renilla luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   FOXO Renilla reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCMV-intron myc Rab6WT

   Plasmid

   #46781

   Purpose

   expression of WT Rab6a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab6 (RAB6A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-mKate2-KDEL-pA

    Plasmid

    #109546

    Purpose

    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-N2-PKCgamma

    Plasmid

    #21204

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKC gamma (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKC gamma (rat). (Lab plasmid WC 0013)

    Promoter

    Available Since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #178087

    Purpose

    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    mScarlet-I mTOR signaling indicator (TOSI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-MCPIP1 (1-599)

    Plasmid

    #127461

    Purpose

    Expressing Flag-tagged human MCPIP1 full lengh protein in mammalian cells

    Depositor

    Mingui Fu

    Article

    Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

    Insert

    MCPIP1 (ZC3H12A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GRK2 S670A

    Plasmid

    #45755

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

    Insert

    GRK2 S670A (GRK2 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S670A

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.2/EV

    Plasmid

    #120833

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2/pr-Membrane

    Plasmid

    #120817

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    pr-Membrane ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBpuro-miRFP670-HRasCT

    Plasmid

    #176030

    Purpose

    Encoding membrane-targeted miRFP670

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    Membrane-targeted miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. opto-Itsn1

    Plasmid

    #174509

    Purpose

    Expresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Itsn1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-Lck-GCaMP2

    Plasmid

    #24794

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

    Insert

    Lck-GCaMP2
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge