We narrowed to 7,279 results for: gnal

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myc-Atg14 S29D

Plasmid

#86757

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

S29D

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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290 RFP/CFP

Plasmid

#83322

Purpose

fluorescent coding joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt6-V5

Plasmid

#35932

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (567) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt5A Non-STOP

Plasmid

#35893

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 mouse twisted gastrulation

Plasmid

#25778

Depositor

Edward De Robertis

Insert

twisted gastrulation (Twsg1 Mouse)

Tags

Flag and chordin signal peptide

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)

Plasmid

#78516

Purpose

Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

LAT (LAT Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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GST-FAST-FH

Plasmid

#16524

Depositor

Bert Vogelstein

Article

Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

Insert

FAST-1 (FH domain) (FOXH1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-150. Forkhead domain.

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
bArr1-ALFA

Plasmid

#201496

Purpose

Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-ALFA (ARRB1 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-HTR6-RhoA sensor

Plasmid

#189614

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6 (HTR6 Human)

Tags

GFP and mScarlet

Expression

Mammalian

Promoter

Tet3G

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-GRM1-IRES-CyRFP1

Plasmid

#195355

Purpose

mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

GRM1-IRES-CyRFP1

Expression

Mammalian

Promoter

pCAG

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHR pUAS-dGFP pPGK-iRFP

Plasmid

#176530

Purpose

Lentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

dGFP
iRFP

Use

Lentiviral

Expression

Mammalian

Promoter

PGKp and UASp

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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MBC2-null

Plasmid

#162749

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MBC1-null

Plasmid

#162748

Purpose

MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S3-RAB_EKARev

Plasmid

#160727

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S3-RAB_EKARev

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_PTS2

Plasmid

#65676

Purpose

Expresses PTS2-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX mSmo-Cterm

Plasmid

#58702

Purpose

used for anti-mouse Smoothened antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

tac

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt9A-V5

Plasmid

#35937

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation A (543) --> G

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-isoform a

Plasmid

#225447

Purpose

Express EGFP-fusion protein of FXR1 isoform a

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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