We narrowed to 7,002 results for: tet

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA

Plasmid

#231604

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag_codon_optimized-8xAAA

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Pseudoknot

Plasmid

#231593

Purpose

Express circular RNA translation reporter with 2xALFATag-10xSunTag-Pseudoknot under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-2xALFATag-10xSunTag-Pseudoknot sequence

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225649

Purpose

tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA, araBAD and LacO3O1 in tandem conformation

Tags

mCherry

Expression

Bacterial

Promoter

tetA, araBAD and LacO3O1 promoters

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2-del-N-ZF(287-342)

Plasmid

#193068

Purpose

doxycycline-inducible expression of mouse Gata2 without the N-terminal zinc finger (amino acids 287-342), in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2-del-N-ZF(287-342)

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2-del-C-ZF(343-379)

Plasmid

#193069

Purpose

doxycycline-inducible expression of mouse Gata2 without the C-terminal zinc finger (amino acids 343-379), in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2-del-C-ZF(343-379)

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2-del-N-Terminal(1-235)

Plasmid

#193067

Purpose

doxycycline-inducible expression of mouse Gata2 without the N-terminal (amino acids 1-235), in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2-del-N-Terminal(1-235)

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186710

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Promoter

Ptrc.x.tetO2

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo

Plasmid

#179440

Purpose

Expresses rTetR-HDAC4(human) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-HDAC4

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-EnR-MCS_N-term

Plasmid

#136884

Purpose

Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-VP64-MCS_N-term

Plasmid

#136886

Purpose

Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

VP64 activator domain

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

Plasmid

#136888

Purpose

Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

KRAB repressor domain

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

Plasmid

#121804

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

Plasmid

#121808

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L900A(pL91)

Plasmid

#124395

Purpose

E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L900A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L900A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898E(pL91)

Plasmid

#124394

Purpose

E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898E (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898E

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898A(pL91)

Plasmid

#124393

Purpose

E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L897A(pL91)

Plasmid

#124392

Purpose

E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L897A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L897A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-I894A(pL91)

Plasmid

#124391

Purpose

E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-I894A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

I894A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)

Plasmid

#70733

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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