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Plasmid#156443PurposeFor transient expression of mouse BORIS (CTCFL) tagged with eGFPDepositorInsertmouse BORIS (Ctcfl Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP
Plasmid#153071PurposeLentiviral vector expressing human ZC3H12D mutated (D95N)DepositorInsertMutated ZC3H12D (D95N) (ZC3H12D Human)
UseLentiviralTagsExpressionMammalianMutationChanged D95 to N, known to abrogate the RNase enz…PromoterEF1αAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin
Plasmid#134863PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-ABEmax-P2A-EGFP-ires-puro
Plasmid#121170PurposeCAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.DepositorInsertABEmax-P2A-EGFP-ires-puro
UseCRISPRTagsP2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP
Plasmid#122954PurposeLentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expressionDepositorInsertNOTCH2NLB (NOTCH2NLB Human)
UseLentiviralTags6xhis tagExpressionMutationPromoterCAG promoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)
Plasmid#70198PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - BirA*
Plasmid#131132PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-T7Max-UTR1-deGFP-8xHis-T500
Plasmid#178422PurposeExpression of deGFP via a T7 Promoter in TXTLDepositorInsertdeGFP
UseSynthetic BiologyTags8xHis TagExpressionBacterialMutationPromoterT7MaxAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP
Plasmid#111518PurposeElectrophysiology in HEK cellDepositorInsertcalmodulin [Xenopus laevis] (calm2 Frog)
UseTagsExpressionMammalianMutationD20A/D56AD93A/D129APromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-iRFP713(V256C)-P2A-EGFP
Plasmid#198071PurposeThe AAV vector for iRFP713(V256C) and EGFPDepositorInsertiRFP713(V256C)
UseAAVTagsP2A-EGFPExpressionMutationV256C mutationPromoterCMVAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP RT-D185K/D186L
Plasmid#101332PurposepBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralTagsExpressionMutationreverse transcriptase D185K/D186LPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-LckAPEX-P2A-EGFP
Plasmid#182826PurposeCre-dependent expression of membrane localized APEXDepositorInsertLckAPEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2
Plasmid#194515PurposeP2 promoter for transgenic enhancer assays in zebrafishDepositorInsertEnhanced Green Fluorescent Protein
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-eno2:Tau-IRES-egfp
Plasmid#112205PurposeUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterDepositorInsertMAPT (MAPT Human)
UseZebrafish expressionTagseno2 exon 2 translational fusion, ires-egfp at c …ExpressionMutationPromotereno2 from zebrafishAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PLtetO-1-UTR1-TetR-deGFP-T500
Plasmid#45774DepositorInserttetR-deGFP
UseSynthetic BiologyTagsFusion protein of TetR and deGFP, with glycine-se…ExpressionBacterialMutationPromoterPLtetO-1Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pM124: pAAV-EFS-CasRx-VEGFA presgRNA
Plasmid#166872PurposeAAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editingDepositorInsertsU6-VEGFA presgRNA
RfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationPromoterEFS and U6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1a-Archon2-KGC-EGFP-ER2
Plasmid#108426PurposeLentivirus production plasmid encoding for Archon2 fluorescent voltage reporterDepositorInsertArchon2-KGC-EGFP-ER2
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)
Plasmid#163044PurposeCheRiff-eGFP channelrhodopsin (plasma membrane)DepositorInsertCheRiff
UseLentiviralTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65
Plasmid#32569DepositorInsertsEGFP
Gal4p65
UseLentiviralTagsExpressionMutationPromoter2TPHAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE
Plasmid#218760PurposeProvides AND intersectional (Cre+Flp) expression of ArchT-eGFPDepositorInsertArchT
UseAAVTagseGFPExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRS-Leader-SARS-CoV-2-EGFP
Plasmid#171585PurposeExpression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporterDepositorInsertTRS-Leader-SARS-CoV-2-d2eGFP
UseLentiviralTags2PEST AND NLSExpressionMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV8-hSyn-flex-miR30-eGFP-shCrh
Plasmid#132715PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factorDepositorInsertGFP-shCrh (Crh Rat)
UseAAVTagsExpressionMutationPromoterSynapsin 1Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L
Plasmid#122991PurposeExpresses Flag-tagged hRPGRIP1L in mammalian cellsDepositorInsertRPGRIP1L (RPGRIP1L Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1 alphaAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Archon1-KGC-EGFP-ER2
Plasmid#108419PurposeAAV production plasmid encoding for Archon1 fluorescent voltage reporterDepositorInsertArchon1-KGC-EGFP-ER2
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-Full-length
Plasmid#194550PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)
Plasmid#156407PurposepCS2 CMV expression vector driving EGFP fused human KRAS4B-G12VDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseTagsEGFPExpressionMammalianMutationmutant KRAS4B-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Flag-Cdk9-D167N-IRES-eGFP
Plasmid#28098DepositorInsertCdk9 (CDK9 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationContains D167N mutation in Cdk9PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTY153 EGFP-T2A-ORF6 SARS-CoV-2
Plasmid#204975PurposeCo-express EGFP and SARS-CoV-2 ORF6DepositorInsertOpen Reading Frame 6 (ORF6 SARS-CoV-2)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTY152 EGFP-T2A-ORF6 SARS-CoV-1
Plasmid#204974PurposeCo-express EGFP and SARS-CoV-1 ORF6DepositorInsertOpen Reading Frame 6 (ORF6 SARS-CoV-1)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG
Plasmid#69842PurposeThis plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationK41M-S285A-T289APromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR
Plasmid#195853PurposeExpresses inhibitory opsin gtACR2 and GFP in turning associated neuronsDepositorInsertGuillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
UseTagsExpressionWormMutationPromoterlim-4Available sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE
Plasmid#89607PurposeExpression of LIN28A in human cellsDepositorInsertLIN28A (LIN28A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterMNDU3Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a-scrambled)
Plasmid#79671PurposeScrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)
Plasmid#191160PurposeExpression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre outputDepositorInsertmCherry:EGFP.s2:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)
Plasmid#114212PurposeLentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortexDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP
Plasmid#175521PurposeNuclear expression of HaloTag10 in mammalian cellsDepositorInsertH2B-HaloTag10
UseTagsExpressionMammalianMutationHaloTag10 = HaloTag7-Q165WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
nCas9-UGI-NLS-P2A-EGFP (pJUL1001)
Plasmid#123611PurposeCAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).DepositorInsertnCas9-UGI-NLS-P2A-EGFP
UseTagsExpressionMammalianMutationD10A in SpCas9PromoterCAGAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only