We narrowed to 159,238 results for: Gene

1. RCAS myr-c-P3k

   Plasmid

   #196373

   Purpose

   Oncogenic with addition of a myristylation signal and expresses in avian cells

   Depositor

   Peter Vogt

   Article

   Chang et al Science. 1997 Jun 20;276(5320):1848-50.

   Insert

   Myr-c-P3k-FLAG
   

   Use

   Retroviral; Avian

   Tags

   flag and myristoylation

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NAT-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193314

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Use

   Tags

   4MYC

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193315

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Use

   Tags

   4FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HYG-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193316

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Use

   Tags

   4MYC

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C-SWAT GFP CL1 DAmP

   Plasmid

   #182513

   Purpose

   C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   CL1 and DAmP
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GB2888

   Plasmid

   #193126

   Purpose

   A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP (A2) G1ab.4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GB2886

   Plasmid

   #193124

   Purpose

   A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP (A2) G1ab.3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR3_BC2

   Plasmid

   #191857

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GB2883

   Plasmid

   #193120

   Purpose

   A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP (A2) G1aG2b.4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GB2882

    Plasmid

    #193119

    Purpose

    A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1aG2b.3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB2878

    Plasmid

    #193115

    Purpose

    A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1aG2b.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GB3270

    Plasmid

    #193114

    Purpose

    Random sequence R3 of 1240 bp for A1 distal promoter position.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A1) Random Sequence R3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GB2881

    Plasmid

    #193118

    Purpose

    A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1aG2b.2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GB3269

    Plasmid

    #193113

    Purpose

    Random sequence R2 of 1240 bp for A1 distal promoter position.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A1) Random Sequence R2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GB2815

    Plasmid

    #193112

    Purpose

    Random sequence R1 of 1240 bp for A1 distal promoter position.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A1) Random Sequence R1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GB2887

    Plasmid

    #193125

    Purpose

    A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1ab.6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GB3275

    Plasmid

    #193129

    Purpose

    A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1abc.2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GB3276

    Plasmid

    #193130

    Purpose

    A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1abc.3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GB3271

    Plasmid

    #193121

    Purpose

    A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1aG2b.7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GB2884

    Plasmid

    #193122

    Purpose

    A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1ab.2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GB3274

    Plasmid

    #193136

    Purpose

    A2 Proximal promoter sequence consisting of the target sequences for the gRNA3 (GB1724) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G3aG2b.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GB2889

    Plasmid

    #193127

    Purpose

    A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1ab.5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GB3272

    Plasmid

    #193134

    Purpose

    A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "b site" (-210 from TSS).

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1b.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GB2891

    Plasmid

    #193128

    Purpose

    A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1abc.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GB2885

    Plasmid

    #193123

    Purpose

    A2 Proximal promoter sequence consisting of two times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1ab.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GB2879

    Plasmid

    #193116

    Purpose

    A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1aG2b.5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GB3278

    Plasmid

    #193132

    Purpose

    A2 Proximal promoter sequence consisting of three times the target sequence for the gRNA1 (GB1838) flanked by random sequences.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1abc.5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GB3273

    Plasmid

    #193135

    Purpose

    A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "e site" (-320 from TSS).

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP (A2) G1e.1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRISPR3_BC6

    Plasmid

    #191861

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRISPR3_BC5

    Plasmid

    #191860

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCRISPR3_BC4

    Plasmid

    #191859

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCRISPR3_BC3

    Plasmid

    #191858

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCRISPR3_BC1

    Plasmid

    #191856

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLTR-DuvSAF

    Plasmid

    #190914

    Purpose

    Pseudotyping of lentiviral vectors

    Depositor

    Jakob Reiser

    Article

    Federici et al Genet Vaccines Ther. 2009 Jan 13;7:1. do

    Insert

    Lyssavirus duvenhage antigenic glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HIV-1 LTR

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKC530 pBABE EGFP-ZNF335

    Plasmid

    #192698

    Purpose

    Retroviral transgene expression of human ZNF335

    Depositor

    Iain Cheeseman

    Article

    Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

    Insert

    ZNF335 (ZNF335 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR

    Plasmid

    #189943

    Purpose

    Lentiviral vector to co-express an spsgRNA with NLS-tagBFP

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT7-EGFP-C1-HsNanos1-del213-267_X

    Plasmid

    #147952

    Purpose

    Mammalian Expression of HsNanos1-del213-267

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos1-del213-267 (NANOS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pT7-V5-SBP-C1-HsNanos1-del1-58_X

    Plasmid

    #147955

    Purpose

    Mammalian Expression of HsNanos1-del1-58

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos1-del1-58 (NANOS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT7-V5-SBP-C1-HsNanos1-del40-56_X

    Plasmid

    #147956

    Purpose

    Mammalian Expression of HsNanos1-del40-56

    Depositor

    Elisa Izaurralde

    Article

    Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

    Insert

    HsNanos1-del40-56 (NANOS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. T7-TetO-GFP_ColE1

    Plasmid

    #173193

    Purpose

    Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-TetO
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. T7-10-QUAS-GFP_ColE1

    Plasmid

    #171657

    Purpose

    QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-10-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. T7-15-QUAS-GFP_ColE1

    Plasmid

    #171658

    Purpose

    QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-15-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. T7-TetO-QF_T7-LacO-TetR_p15A

    Plasmid

    #171659

    Purpose

    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-TetR-T7 Stop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. QUAS-0-T7-GFP_ColE1

    Plasmid

    #171649

    Purpose

    QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-0-T7
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. T7-0-QUAS-GFP_ColE1

    Plasmid

    #171650

    Purpose

    QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-0-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. QUAS-0-T7-CcdB_ColE1

    Plasmid

    #171652

    Purpose

    QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    CcdB-ssrA
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. QUAS-10-T7-GFP_ColE1

    Plasmid

    #171654

    Purpose

    QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-10-T7
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. QUAS-15-T7-GFP_ColE1

    Plasmid

    #171655

    Purpose

    QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-15-T7
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. T7-5-QUAS-GFP_ColE1

    Plasmid

    #171656

    Purpose

    QUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-5-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMLS640

    Plasmid

    #188892

    Purpose

    ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9, Cbr-unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge