We narrowed to 75,842 results for: Rest

1. pErCas12a EZ Clone

   Plasmid

   #132641

   Purpose

   Expresses ErCas12a and an sgRNA to target a region of interest

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   ErCas12a
   

   Use

   AAV and CRISPR

   Tags

   3x HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Q926R (Please see depositor comments) and BsaI si…

   Promoter

   CMV Promoter

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TGFB1_pLX307

   Plasmid

   #98377

   Purpose

   Lentiviral expression of TGFB1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TGFB1 (TGFB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. ALCAM-p2A-E2Crimson

   Plasmid

   #83939

   Purpose

   Lentiviral vector expressing ALCAM-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   ALCAM (ALCAM Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CDK9_pLX307

   Plasmid

   #98324

   Purpose

   Lentiviral expression of CDK9

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   CDK9 (CDK9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

   Plasmid

   #49077

   Purpose

   Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x HA and mCerulean

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00035 (aka pMG0784)

   Plasmid

   #210768

   Purpose

   Lentiviral vector to express human ADRM1

   Depositor

   Raj Chari

   Article

   Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

   Insert

   ADRM1 (ADRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TUBG1_pLX307

   Plasmid

   #98376

   Purpose

   Lentiviral expression of TUBG1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   TUBG1 (TUBG1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSecTaq2-hGas6-Myc-6xHis

   Plasmid

   #226523

   Purpose

   Expresses TAM Receptor Ligand human GAS6

   Depositor

   Sergei Kotenko, Raymond Birge

   Article

   Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

   Insert

   Human GAS6 (GAS6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 (NT17)

   Plasmid

   #49085

   Purpose

   Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available since

   Nov. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87829

    Purpose

    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    MTS
    NLS
    PTS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_RHEB_WT

    Plasmid

    #81257

    Purpose

    Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIG-FGFRdn

    Plasmid

    #80431

    Purpose

    Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Fibroblast growth factor receptor 1 (FGFR1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa 1–425

    Promoter

    beta-actin promoter

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

13. LIMD1_pLX307

    Plasmid

    #98349

    Purpose

    Lentiviral expression of LIMD1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    LIMD1 (LIMD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_NMNAT3_WT

    Plasmid

    #81885

    Purpose

    Gateway Donor vector containing NMNAT3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NMNAT3 (NMNAT3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_TRAF5_WT

    Plasmid

    #82167

    Purpose

    Gateway Donor vector containing TRAF5 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TRAF5 (TRAF5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. DDX39B_pLX307

    Plasmid

    #98331

    Purpose

    Lentiviral expression of DDX39B

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    DDX39B (DDX39B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S296P

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV.EF1α.mEmerald.CD9.miR9T

    Plasmid

    #170452

    Purpose

    Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.

    Depositor

    Tsuneya Ikezu

    Article

    Clayton et al Mol Neurodegener. 2021 Mar 22;16(1):18.

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP71-HLA-B0702-His

    Plasmid

    #104850

    Purpose

    HLA-B0702 his tagged

    Depositor

    Sébastien Walchli

    Article

    Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Retroviral

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. JUP_pLX307

    Plasmid

    #98347

    Purpose

    Lentiviral expression of JUP

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    JUP (JUP Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pODN-mNG-ANLN

    Plasmid

    #183834

    Purpose

    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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