We narrowed to 9,681 results for: SUB;
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Plasmid#104547PurposeExpresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.DepositorInsertCry2-mCherry-PKA W196R/F327A
TagsmCherryExpressionMammalianMutationPKA W196R and Y204APromoterCMVAvailable SinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR01-FnCpf1
Plasmid#176529PurposeNuclease Cpf1 adapted for Aspergillus speciesDepositorInsertFnCpf1
UseCRISPR; Fungal expressionPromoterTEF1Available SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS585P-myc
Plasmid#107498PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotypeDepositorAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhBΔPIP
Plasmid#108696PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletionDepositorInsertrnhB
TagsGSTExpressionBacterialMutationC-terminal truncation (7aa) removing PIP boxPromotertacAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhB D101N
Plasmid#108695PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutationDepositorInsertrnhB
TagsGSTExpressionBacterialMutationD101N catalytic site mutationPromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716Available SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALNL-DCX-eGFP
Plasmid#28310DepositorAvailable SinceApril 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJMP1274
Plasmid#119266PurposeB. subtilis deltaTn7 site and flanking sequence cloned into pACYCDepositorInsertB. subtilis deltaTn7
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1263
Plasmid#119264PurposeB. subtilis attTn7 site and flanking sequence cloned into pACYCDepositorInsertB. subtilis attTn7
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZE43 Dapper-2 pCS2P+
Plasmid#17084DepositorInsertDapper-2 (dact2 Zebrafish)
UseZebrafish expressionAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-human MYCN-V421D
Plasmid#74166PurposeMammalian expression of human MycN-VD mutantDepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo S100a10 in situ probe
Plasmid#45603DepositorInserts100a10 in situ probe (S100a10 Mouse)
UseIn situMutationfragment contains bp# 21-275 of BC025044Available SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC
Plasmid#99649PurposeBacterial expression of wildtype Rag GTPase dimerDepositorTags10x His tag, Poly arginine linker, and SUMOExpressionBacterialPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 deltaP
Plasmid#8596DepositorInsertSHP1 (PTPN6 Human)
TagsGSTExpressionBacterialMutationdeltaP (deletion of phosphatase domain, amino aci…Available SinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046952
Plasmid#145718PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046863
Plasmid#145682PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046938
Plasmid#145599PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pILGFP86
Plasmid#83543PurposeUsed to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.DepositorInsertRPL4A promoter-yEGFP
ExpressionYeastPromoterRPL4AAvailable SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRExpressionBacterialMutationD917A (nuclease-deactivating)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only