We narrowed to 7,200 results for: mme

1. Pb459-mU6-sgRNA-EF1a-PuroR

   Plasmid

   #195507

   Purpose

   piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   Puromycin
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pISceI Dusp6:memGFP

   Plasmid

   #32668

   Purpose

   Expresses membrane localized EGFP under the Dusp6 promoter

   Depositor

   Michael Tsang

   Article

   Wang et al Development. 2011 Jan;138(1):45-54. Epub

   Insert

   Dusp6 promoter (dusp6 Zebrafish)
   

   Use

   Zebrafish transgenesis

   Available Since

   Nov. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-mCherry::LANS1

   Plasmid

   #60784

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS1
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_CRISPRi_sgNeg1

   Plasmid

   #166998

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. attB-­GFP-­Poly(A)

   Plasmid

   #65524

   Purpose

   Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGK:TRL-EFS:iRFP670

   Plasmid

   #68475

   Purpose

   Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   iRFP670
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   EFS and pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-ERK5kin

   Plasmid

   #65245

   Purpose

   Expression of HA tagged ERK5 kinase domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

   Insert

   ERK5kin (MAPK7 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 410-817

   Promoter

   CMV

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCGN-ELK-1 Ets mut

   Plasmid

   #27157

   Purpose

   For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   ELK1 (ELK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R62E/R65E/Y66D

   Available Since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. mRIP3 GFP D161N

   Plasmid

   #41383

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP3 (Ripk3 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (D161N)

   Promoter

   CMV

   Available Since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. mADAM17deltaMP in pQCXIP cFLAG

    Plasmid

    #19144

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 189-378

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pCDH-EF1a-tetR-T2A-iRFP

    Plasmid

    #176028

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    iRFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TF-bio-His

    Plasmid

    #52184

    Purpose

    Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TF (TF Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-GW-dCas9-SET2-IRES2-mCherry

    Plasmid

    #202089

    Purpose

    Neuron-specific expression vector of dCas9-Set2

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2
    

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJCC_075 AsCas12a D908A

    Plasmid

    #171668

    Purpose

    For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a D908A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 908 to alanine

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pegRNA_HEK4_20-7

    Plasmid

    #201974

    Purpose

    Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_20-7
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pROF394

    Plasmid

    #155333

    Purpose

    Expression of PULSE-controlled LFY-VP16-2A-RLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
    

    Use

    Synthetic Biology; Eukaryotic expression

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-CVm-PTEN

    Plasmid

    #168032

    Purpose

    Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-PTEN(M265-N329)-mVenus
    

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_PGK_SNIPR_synRobo/Notch

    Plasmid

    #188341

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_Robo1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSET-TEV-12His

    Plasmid

    #137032

    Purpose

    empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Type

    Empty backbone

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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