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LENTICRISPR-TPI1_sgRNA1

Plasmid

#201626

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TPI1_sgRNA2

Plasmid

#201627

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

TPI1 (TPI1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 S454A

Plasmid

#19844

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S454A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 454 to Alanine

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-MYC-ITFG2

Plasmid

#87046

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

Plasmid

#83453

Purpose

Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

polycystin-1 (Pkd1 Mouse)

Tags

2xMyc, 3xFlag, and 6xHis

Expression

Mammalian

Mutation

S4144D

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon9 E545K (pFastBac)

Plasmid

#16640

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

E545K

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf37

Plasmid

#12725

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf37

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf48

Plasmid

#12736

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf48

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
YFP-Rpp40-C1

Plasmid

#134549

Purpose

Expresses YFP-Rpp40 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp40 (RPP40 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-MAVS-5'UTR

Plasmid

#184639

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET-22HT-MSI2(8-193)

Plasmid

#60356

Purpose

Express the RBD of human MSI2 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

human MSI2 (8-193) (MSI2 Human)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 8-193 of MSI2

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf42

Plasmid

#12730

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf42

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAcrF2

Plasmid

#89234

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF2

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGAM1_sgRNA2

Plasmid

#201615

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PGAM1 (PGAM1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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PAK7_HUMAN_D0

Plasmid

#79706

Purpose

This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK7 (PAK5 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCMV10-Flag-Fbxl3

Plasmid

#48751

Purpose

Expression of FLAG-tagged Fbxl3

Depositor

Joseph Takahashi

Article

Siepka et al Cell. 2007 Jun 1;129(5):1011-23. Epub 20

Insert

Fbxl3 (Fbxl3 Mouse)

Tags

3XFLAG

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
MK13_HUMAN_D0

Plasmid

#79693

Purpose

This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK13

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf196

Plasmid

#12884

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf196

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
PIK3CA - Exon20 H1047R (pFastBac)

Plasmid

#16641

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf117

Plasmid

#12805

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf117

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only