We narrowed to 13,031 results for: Hal

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pFGC5941-PacI-ath-STTM165/166-48nt

Plasmid

#84091

Purpose

target miRNA165/166 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM165/166

Expression

Plant

Promoter

2x35S

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1-DN

Plasmid

#109582

Purpose

Multisite gateway vector for 3' tagging with dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p4305

Plasmid

#198150

Purpose

bacterial expression of sifA::HaloTag fusion

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)

Tags

HaloTag-HA

Expression

Bacterial

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1

Plasmid

#109581

Purpose

Multisite gateway vector for 3' tagging with Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMC_mNG2(11)_BSDminus_F2

Plasmid

#184164

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTS26_QUAS_CsChrimson

Plasmid

#175548

Purpose

Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

Depositor

Leslie Vosshall

Article

Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

Insert

CsChrimson

Tags

tdTomato

Expression

Insect

Promoter

QUAS

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMP416

Plasmid

#134996

Purpose

dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

Depositor

Ahmad Khalil

Article

Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

Insert

pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

Use

Synthetic Biology

Tags

3xFLAG, E coli Dam(N132A), and dCas9

Expression

Mammalian

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE1109

Plasmid

#202773

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1

Plasmid

#109529

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1-DN

Plasmid

#109530

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-KDEL-pA

Plasmid

#109546

Purpose

Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMRS-AraC-GFP

Plasmid

#220199

Purpose

mini-Tn7 GFP expression vector - AraC/pBAD promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

AraC/pBAD

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-XylS-GFP

Plasmid

#220200

Purpose

mini-Tn7 GFP expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

XylS/pM

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR4 TNRC6B

Plasmid

#20022

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6B (TNRC6B Human)

Use

Gateway Cloning

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pTpal-fdc

Plasmid

#78288

Purpose

Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

Inserts

PAL2 (PAL2 Mustard Weed)
FDC1 (FDC1 Budding Yeast)

Expression

Bacterial

Mutation

*see below

Promoter

trc

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-aZEA

Plasmid

#53286

Purpose

Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

lcyE (LUT2 Mustard Weed)

Use

Low copy number bacterial cloning vector

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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actb2-SecAnxaV-mKate2-acry-EGFP

Plasmid

#123628

Purpose

Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

AnnexinA5

Tags

mKate2

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pZA26

Plasmid

#158504

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

D489V

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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