We narrowed to 11,437 results for: 112

1. pUDE1084

   Plasmid

   #168500

   Purpose

   expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

   Depositor

   Jean-Marc Daran

   Article

   Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

   Inserts

   Fncas12a
   T7RNA polymerase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TDH3 and TEF1

   Available since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBR322-Pdp1_NTD-ZFD_R

   Plasmid

   #198277

   Purpose

   For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-ZFD_R
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDSHA-SARA-Myc

   Plasmid

   #67368

   Purpose

   Mammalian expression of hSara with C-term Myc tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pED-hIRE1(I642G)

   Plasmid

   #16263

   Depositor

   Peter Walter

   Article

   Lin et al Science. 2007 Nov 9. 318(5852):944-9.

   Insert

   IRE1 (ERN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   isoleucine to glycine mutation at residue 642

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_SNX18-1/1

   Plasmid

   #91369

   Purpose

   Protein expression and purification of human SH3 domain construct SNX18-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SNX18-1/1 (SNX18 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFlox_N-p300_BSD_FKBPF36V_EYFP

   Plasmid

   #163885

   Purpose

   Acute Depletion P300-EYFP

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   P300 (Ep300 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti_CRISPRi_sgNeg1

   Plasmid

   #166998

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH6_new

   Plasmid

   #105866

   Purpose

   more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

   Depositor

   Andreas Weber

   Article

   Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

   Insert

   U6-26p::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Subcloning

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-LacI-p300

   Plasmid

   #179270

   Purpose

   Expresses GFP-LacI-p300 fusion plasmid

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Methods Mol Biol. 2023;2589:361-376. doi

   Insert

   E1A binding protein p300 (EP300 Human)
   

   Use

   Tags

   GFP-LacI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFPH7

    Plasmid

    #83554

    Purpose

    Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    ADH2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mRIP3 GFP D161N

    Plasmid

    #41383

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (Ripk3 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Kinase Domain Mutant (D161N)

    Promoter

    CMV

    Available since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pEW4: pET-32a Dot1L(2-386)

    Plasmid

    #124099

    Purpose

    Dot1L(2-386) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-386) (DOT1L Human)
    

    Use

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFlox_N-p300_Neo_FKBPF36V_mScarlet

    Plasmid

    #163886

    Purpose

    Acute Depletion of P300-mScarlet

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    P300 (Ep300 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GADD45 P-2/3m-luc

    Plasmid

    #8359

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter P-2/3m (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    P-2/3m

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pHH0103_PACSIN2-1/1

    Plasmid

    #91330

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN2-1/1 (PACSIN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pILGFP97

    Plasmid

    #83551

    Purpose

    Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CYC1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSiren_EGFP-shHmga1#3

    Plasmid

    #122289

    Purpose

    Knockdown for mouse Hmga1

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Knockdown shRNA for mouse Hmga1 (Hmga1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBR322-Pdp1_NTD-ZFN_L-HiBiT

    Plasmid

    #198319

    Purpose

    Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-ZFN_L-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL2

    Plasmid

    #48742

    Purpose

    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    truncated mPer2 promoter/enhancer region (-1128 to +386)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    truncated promoter region (see pGL6)

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pHA-ΔN335-Kin1A

    Plasmid

    #31606

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN335 kinesin-1A (Kif5a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-335

    Promoter

    CMV

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only