We narrowed to 5,294 results for: codon optimized

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pCEP4-gp120(BaL)-CD4

Plasmid

#98957

Purpose

Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

HIV-1 (BaL) gp120

Tags

CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-st1CAS9-pA

Plasmid

#131542

Purpose

Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

Insert

Codon optimized st1Cas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pVRc22_4450

Plasmid

#49741

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS22_4450

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-ODC1

Plasmid

#133256

Purpose

Express FLAG-tagged ODC1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

ODC1 (ODC1 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Sox17(2A-phiC31o)

Plasmid

#140762

Purpose

Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

phiC31o

Use

Mouse Targeting

Promoter

Endogenous Sox17

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A-C431S

Plasmid

#45977

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A-C431S (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Doub…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)-strep

Plasmid

#180023

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)-Strep

Plasmid

#164444

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Proline 801 to Glycine

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-2xAM_hIRF-1

Plasmid

#92221

Purpose

Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Inserts

Sp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter

Use

CRISPR and Lentiviral

Tags

2xAM tag

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

CBh and U6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pVRa34_1384

Plasmid

#49693

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF34_1384

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pJR2953

Plasmid

#122549

Purpose

Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemCardinal2

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pVRa12_807

Plasmid

#49663

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF12_807

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A

Plasmid

#129679

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0202

Plasmid

#185626

Purpose

MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

SpCas9

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:mVenus

Plasmid

#133970

Purpose

Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pVRa11_987

Plasmid

#49661

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF11_987

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Gamma 5

Plasmid

#42200

Depositor

Narasimhan Gautam

Article

Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

Insert

Gamma 5 (Gng5 Rat)

Tags

Venus(1-155)

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Q842gp120-8his

Plasmid

#100932

Purpose

Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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