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p21 promoter E

Plasmid

#16466

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (L135V)

Plasmid

#31154

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Leucine 135 changed to Valine

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (E87R)

Plasmid

#31152

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glutamic acid 87 changed to Arginine

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (G244R)

Plasmid

#31155

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glycine 244 changed to Arginine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC3

Plasmid

#72712

Purpose

Lentiviral expression of shRNA against CRTC3 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC3

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3HA-AMOT p130∆PPxY1

Plasmid

#166711

Purpose

expresses AMOT with inactivating mutation in first PPxY motif in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 L129A P130A

Tags

2xHA

Expression

Mammalian

Mutation

changed leucine 129 to alanine, proline 130 to al…

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

Plasmid

#149590

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFAB822

Plasmid

#47856

Purpose

BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

BBa_B1006 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO11

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB803

Plasmid

#47847

Purpose

BIOFAB reporter plasmid for measuring T7 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T7 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO2

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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DsRed2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176988

Purpose

Mammalian expression vector encoding DsRed2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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minitalin (headR13DD)

Plasmid

#182994

Purpose

expression of minitalin wt in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

minitalin (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET41a iGluf

Plasmid

#119833

Purpose

Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluf

Tags

GST

Expression

Bacterial

Mutation

E25D

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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T7-His-GFP-Arp11

Plasmid

#51402

Purpose

expression of GFP-tagged mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Tags

GFP and His

Expression

Bacterial

Promoter

T7

Available Since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC1

Plasmid

#72710

Purpose

Lentiviral expression of shRNA against CRTC1 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC1

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
p21 promoter C

Plasmid

#16464

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pOmpT

Plasmid

#112123

Purpose

Bacterial expression outer membrane protein T (OmpT), wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

OmpT (ompT E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pR-BAM

Plasmid

#22649

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1L (Myt1l Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pCSC-ASCL1-IRES-Sox11

Plasmid

#182234

Purpose

To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

Depositor

Chun-Li Zhang

Article

Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

Insert

ASCL1-IRES-Sox11

Use

Lentiviral

Promoter

CMV

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Vkg::mCherry

Plasmid

#163929

Purpose

Expression of GrabFP-ECM under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

G1767D

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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