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pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

Plasmid

#129180

Purpose

Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-IGK-dAPEX2-KDEL

Plasmid

#117183

Purpose

Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

IGK-dAPEX2-KDEL

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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DRH031_ AAV-SIO-nEF-WT-THR

Plasmid

#225090

Purpose

Cre-dependent expression of wild-type thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

DN-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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DRH032_ AAV-SIO-nEF-DN-THR

Plasmid

#225091

Purpose

Cre-dependent expression of dominant-negative thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

WT-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-FLPo-T2A-GFP

Plasmid

#161766

Purpose

express FLPo and GFP under the control of human synapsin promoter

Depositor

Baoji Xu

Article

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Inserts

FLPo
T2AEGFP

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hMECP2-cycle3GFP

Plasmid

#163706

Purpose

pAAV plasmid for Cre-dependent expression of human MECP2 fused with cycle3 GFP under Syn promoter

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

hMECP2-cycle3GFP (MECP2 Aequorea victoria, Human)

Use

AAV

Promoter

hSyn

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-oG-WPRE-hGH

Plasmid

#74290

Purpose

pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG (optimized Glycoprotein)

Use

AAV

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

Ef1a

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE

Plasmid

#198514

Purpose

Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CaMKIIα minimal promotor (0.4kb)

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP680-P2A-GFP

Plasmid

#197164

Purpose

Expresses the protein of miRFP680-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FLEX-ChR2-YFP

Plasmid

#110337

Purpose

ChR2-YFP reporter vector of iTango and Cal-Light system for AAV production

Depositor

Hyungbae Kwon

Insert

tetO-Flex-ChR2-YFP

Use

AAV

Expression

Mammalian

Mutation

Chr2 H134R

Promoter

tetO

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

Plasmid

#39454

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CD (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230010

Purpose

Expresses anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-Chrimson-TdTomato

Plasmid

#209202

Purpose

Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics

Depositor

Larry Zweifel

Article

Soden et al Nat Neurosci. 2020 Aug;23(8):968-980. do

Insert

Chrimson

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-oChIEF-tdTomato

Plasmid

#30541

Purpose

Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

Depositor

Rory McQuiston

Article

Bell et al Neuropharmacology. 2011 Aug 25.

Insert

oChIEF-tdtomato

Use

AAV and Cre/Lox

Tags

tdTomato

Promoter

CAG

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-WPRE

Plasmid

#202611

Purpose

Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(FL)

Plasmid

#230023

Purpose

Expresses anti-mCherry PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-LaM6-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Article

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Promoter

hSyn

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[CoChR-GFP]

Plasmid

#62724

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

Plasmid

#165430

Purpose

Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

FHS-paCaMKII(SD)

Use

AAV

Tags

FHS(Flag, HisX6, Strep) tag

Expression

Mammalian

Mutation

C450A, L514K, G528A, L531E, N538E: Light-insensit…

Promoter

CaMKII0.4

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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