We narrowed to 17,102 results for: emb

1. NKP9 pmRFP-N2 (719)

   Plasmid

   #61999

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf62 (CYBC1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. NKP40 RFP (794)

   Plasmid

   #62006

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf32 (C17orf32 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   pgk

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pTH731-2µ-RLuc/minCFLuc

   Plasmid

   #40601

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

   Plasmid

   #236246

   Purpose

   Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Promoter

   U6 and human Synapsin1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

   Plasmid

   #105802

   Purpose

   Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-mRuby3; ORF2: TEV-mClover3

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

   Plasmid

   #214137

   Purpose

   Insect cell expression of the human 20SCP proteasome assembly chaperones

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   POMP (POMP Human)
   PSMG1 (PSMG1 Human)
   PSMG2 (PSMG2 Human)
   PSMG3 (PSMG3 Human)
   PSMG4 (PSMG4 Human)
   

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGM238

   Plasmid

   #220178

   Purpose

   Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   NACA and BTF3 (NACA, BTF3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG (NACA); 6xHis (BTF3)

   Mutation

   K78E (NACA) + K43B (BTF3)

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

   Plasmid

   #224497

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICE-HA-Ku70-siR-Mut6E

   Plasmid

   #82330

   Purpose

   Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mut6E corresponding to K282E K287E T300E K331E K3…

   Promoter

   CMV-tet

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. STT3A pEGFP-N2 (1051)

    Plasmid

    #62025

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pKK-RNAi-nucEGFPmiR-TEV-mCherry

    Plasmid

    #105812

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cherry-HA-KCC2

    Plasmid

    #104077

    Purpose

    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

    Depositor

    Igor Medyna

    Article

    Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Tags

    mCherry-HA tag

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    UbC

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

    Plasmid

    #105810

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

    Plasmid

    #105809

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKK-RNAi-nucEGFPmiR-TEV-FLAG

    Plasmid

    #105811

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTH734-2µ-RLuc/stopCFLuc

    Plasmid

    #40609

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase (nonsense mutant)
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKK-RNAi-nucEGFPmiR-mCherry-TEV

    Plasmid

    #105808

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    mCherry-TEV

    Expression

    Mammalian

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKK-RNAi-nucEGFPmiR-FLAG-TEV

    Plasmid

    #105807

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

    Plasmid

    #192239

    Purpose

    knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid

    Plasmid

    #192242

    Purpose

    knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    eIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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