We narrowed to 9,260 results for: pho

1. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

   Plasmid

   #196185

   Purpose

   Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Inserts

   GSK3B (GSK3B Human)
   λPPase
   

   Use

   Tags

   GST, HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-DroshaS300E/S302D

   Plasmid

   #62531

   Purpose

   Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serine 300 to glutamic acid and serine 3…

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBOBI C-FLAG PHGDH

   Plasmid

   #212995

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mGFP-hNET

   Plasmid

   #193364

   Purpose

   expression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

   Depositor

   Harald Sitte

   Article

   Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

   Insert

   human Norepinephrine Transporter (SLC6A2 Human)
   

   Use

   Tags

   monomeric GFP (mGFP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-hPKL WT

   Plasmid

   #107159

   Purpose

   For mammalian expression of Flag-hPKL WT

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   Flag-hPKL WT (PKLR Human)
   

   Use

   Retroviral

   Tags

   N-term Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcdna6.2- myc ULK1 wt

   Plasmid

   #27629

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 (Myc Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Promoter

   Available since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pGEX4T2-Ste7-EE

   Plasmid

   #52681

   Purpose

   expression of constitutively active Ste7 kinase in bacteria

   Depositor

   Benjamin Turk

   Article

   Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

   Insert

   Ste7 (STE7 Budding Yeast)
   

   Use

   Tags

   GST and Myc

   Expression

   Bacterial

   Mutation

   S359E/T363E constitutively active

   Promoter

   tac

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pFl Strep-sumo-TEV-hAgo2 D597N

   Plasmid

   #136236

   Purpose

   Expressing catalytically inactive human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Use

   Tags

   Sterp Sumo

   Expression

   Insect

   Mutation

   D597N

   Promoter

   polyhedrin

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX SHP-2(N)-SH2

   Plasmid

   #46498

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(N) (PTPN11 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-104

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. MEK1C222

    Plasmid

    #164638

    Purpose

    Bacterial expression plasmid for His6-MEK1C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S222C/C277S/C376S

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX RASGAP(N)-SH2

    Plasmid

    #46432

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Gap/RASGAP(N) (RASA1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 168-283

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1-FH-AGO2-5xA

    Plasmid

    #91979

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX SHP-2(C)-SH2

    Plasmid

    #46497

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(C) (PTPN11 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 101-222

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV-Puro-FH-AGO2-5xE

    Plasmid

    #91993

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-FH-AGO2-5xE

    Plasmid

    #91981

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 - myc ULK1 wt

    Plasmid

    #27623

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Expression

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

17. MSCV-Puro-FH-AGO2-5xA

    Plasmid

    #91992

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV5-Flag-Ago2-FDM3

    Plasmid

    #72210

    Purpose

    Mammalian expression of aa 227-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-226

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pRK5-Myc-PSK2 (1-416)

    Plasmid

    #197123

    Purpose

    Expression of Myc-tagged PSK2 (aa1-416) / TAOK1 (aa1-416) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

    Insert

    TAOK1 (aa1-416) (TAOK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deleted C-terminus

    Promoter

    SV40

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5-Flag-Ago2-FDM1

    Plasmid

    #72208

    Purpose

    Mammalian expression of aa 1-372 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 373-860

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only