We narrowed to 229 results for: Ypet
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Plasmid#118723PurposeThe donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based no force control)
Plasmid#118722PurposeThe donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II acceptor only control (F40-based no force control)
Plasmid#118720PurposeThe acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y67G mutation in YPet(sh…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianPromoterEF-1-alpha promoterAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Fyn FRET Biosensor
Plasmid#124654PurposeFRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPetDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
ExpressionMammalianAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Tre-Rac1-P29S (FLARE)
Plasmid#241371PurposeDox-inducible expression of Rac1-P29S FLARE sensorDepositorUseLentiviralTagsYPet (N terminal to Pak1) and mCerulean3 (N termi…MutationP29S in Rac1PromoterTREAvailable SinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RL016A
Plasmid#42135DepositorInsertmYPet-mCherry
ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-YC
Plasmid#107072PurposeHost vector to construct a YPer or CyPet fusion proteinDepositorInsertYPet-CyPet fusion protein
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-YFP
Plasmid#90104PurposeYFP control expression plasmidDepositorInsertYFP
UseBroad-host rangeExpressionBacterialPromoterPtacAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaNAR2
Plasmid#64728PurposeFRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertCaNAR2
TagsCerulean3 and YPetExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only